O'Connor P M, Zysow B R, Schoenhaus S A, Ishida B Y, Kunitake S T, Naya-Vigne J M, Duchateau P N, Redberg R F, Spencer S J, Mark S, Mazur M, Heilbron D C, Jaffe R B, Malloy M J, Kane J P
Cardiovascular Research Institute, University of California-San Francisco, 94143, USA.
J Lipid Res. 1998 Mar;39(3):670-8.
Prebeta-1 HDL is a molecular species of plasma HDL of approximately 67 kDa mass that contains apolipoprotein A-I, phospholipids, and unesterified cholesterol. It participates in a cyclic process involved in the retrieval of cholesterol from peripheral tissues. In this cycle, unesterified cholesterol from cells is incorporated into prebeta-1 HDL, providing a substrate for esterification of cholesterol by lecithin:cholesterol acyltransferase. Prebeta-1 HDL then becomes incorporated into larger HDL species of alpha mobility as esterification proceeds and is regenerated during the transfer of cholesteryl esters from alpha HDL particles to acceptor lipoproteins. Thus the steady state level of prebeta-1 HDL in plasma reflects the relative efficiencies of the major metabolic processes involved in its generation and removal. We have used an isotope dilution technique to measure prebeta-1 HDL levels in the plasmas of 136 normolipidemic individuals (46 M, 90 F). The mean absolute concentration of prebeta-1 HDL as apolipoprotein A-I was 68 +/- 40 microg/ml for women, and 84 +/- 49 m/ml for men. Prebeta-1 HDL represented 5.5 +/- 3.3% of total apolipoprotein A-I in women, and 7.2 +/- 4.0% in men. The distributions of both absolute and percent prebeta-1 HDL are highly asymmetric, with skew toward higher values. However, the skew appears not to be attributable to either plasma cholesterol or triglyceride levels which are also skewed in population samples. The percent prebeta-1 HDL was negatively correlated with HDL cholesterol levels (P < 0.0001), whereas absolute levels of prebeta-1 HDL were positively correlated with apolipoprotein A-I and negatively correlated with HDL cholesterol (P, for both, < 0.0001). Multiple linear regression analysis revealed effects of age and gender, but no association with lipoprotein fractions other than HDL. Lower levels of prebeta-1 HDL were associated with female gender in all models.
前β-1高密度脂蛋白（Prebeta-1 HDL）是血浆高密度脂蛋白的一种分子形式，质量约为67 kDa，含有载脂蛋白A-I、磷脂和未酯化胆固醇。它参与了从外周组织回收胆固醇的循环过程。在这个循环中，细胞中的未酯化胆固醇被整合到前β-1 HDL中，为卵磷脂：胆固醇酰基转移酶将胆固醇酯化提供底物。随着酯化过程的进行，前β-1 HDL随后被整合到具有α迁移率的更大的高密度脂蛋白种类中，并在胆固醇酯从α高密度脂蛋白颗粒转移到受体脂蛋白的过程中再生。因此，血浆中前β-1 HDL的稳态水平反映了其生成和清除过程中主要代谢过程的相对效率。我们使用同位素稀释技术测量了136名血脂正常个体（46名男性，90名女性）血浆中的前β-1 HDL水平。女性前β-1 HDL作为载脂蛋白A-I的平均绝对浓度为68±40微克/毫升，男性为84±49微克/毫升。前β-1 HDL在女性总载脂蛋白A-I中占5.5±3.3% , 在男性中占7.2±4.0%。前β-1 HDL的绝对含量和百分比分布都高度不对称，偏向较高值。然而，这种偏态似乎不归因于血浆胆固醇或甘油三酯水平，这两者在人群样本中也呈偏态分布。前β-1 HDL百分比与高密度脂蛋白胆固醇水平呈负相关（P < 0.0001），而前β-1 HDL的绝对水平与载脂蛋白A-I呈正相关，与高密度脂蛋白胆固醇呈负相关（两者P均< 0.0001）。多元线性回归分析显示了年龄和性别的影响，但与除高密度脂蛋白外的脂蛋白组分无关。在所有模型中，前β-1 HDL水平较低与女性性别相关。
