Svitashev S, Bryngelsson T, Li X, Wang R R
Department of Plant Breeding Research, Swedish University of Agricultural Sciences, Svalöv, Sweden.
Genome. 1998 Feb;41(1):120-8.
We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St, Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL-GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in various Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pP1Taq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, whose genome formulas were unknown. Our results demonstrate that only three of eight Elymus species examined (the tetraploid species Elymus grandis and the hexaploid species Elymus caesifolius and Elymus borianus) really belong to Elymus.
我们已经开发出了针对在披碱草属物种进化过程中涉及的基因组（即St、Y、H、P和W基因组）的RFLP和RAPD标记。分别从冰草属和绒毛澳披碱草的基因组中分离出了两个P基因组特异性重复DNA序列pAgc1（350 bp）和pAgc30（458 bp），以及三个W基因组特异性序列pAuv3（221 bp）、pAuv7（200 bp）和pAuv13（207 bp）。寻找Y基因组特异性序列的尝试未成功。一级结构分析表明，pAgc1（P基因组）和pAgc30（P基因组）在227 bp的片段上有81%的相似性。这三个W基因组特异性序列也高度同源。序列比较分析显示与EMBL - GenBank数据库中的序列无同源性。为五个基因组中的每一个都找到了三到四个基因组特异性RAPD标记。基因组特异性条带被克隆，并证明主要是存在于各种小麦族物种中的低拷贝序列。所获得的RFLP和RAPD标记，连同先前描述的H和St基因组特异性克隆pHch2和pP1Taq2.5以及Ns基因组特异性RAPD标记，被用于研究一些基因组公式未知的披碱草属物种和欧洲大麦草的基因组组成。我们的结果表明，在所检查的八个披碱草属物种中，只有三个（四倍体物种大披碱草和六倍体物种硬叶披碱草和博氏披碱草）真正属于披碱草属。
