Yoshida E, Iwatsuki K, Miyashita N, Gemma T, Kai C, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Vet Microbiol. 1998 Jan 16;59(2-3):237-44. doi: 10.1016/s0378-1135(97)00194-6.
We analyzed the molecular properties of the nucleocapsid protein (NP) of canine distemper viruses (CDV), isolated between 1992 and 1995 in Japan. Four CDV field isolates (Yanaka, Ueno, Hamamatsu, and Adachi strains) obtained were antigenically identical. Sequence analysis of entire region of the NP gene of a field isolate, the Yanaka strain, revealed that the NP gene contained 1683 nucleotides and was 93.2% homologous with a laboratory strain, the Onderstepoort strain. The deduced amino acid sequence contained 523 amino acids and was 95.2 and 99% homologous with those of the Onderstepoort and a virulent strain, A75/17 strain, respectively. Since most of the diversities in amino acid sequence occurred in two domains, at the N'- and the C'- termini, we further sequenced 3'-terminal regions of the remaining three field isolates. Based on the sequences, the new CDV isolates had one cluster that distinguished them from the laboratory strain.
我们分析了1992年至1995年在日本分离出的犬瘟热病毒（CDV）核衣壳蛋白（NP）的分子特性。获得的4株CDV野外分离株（柳町、上野、滨松和足立株）抗原性相同。对一株野外分离株柳町株NP基因的整个区域进行序列分析，发现NP基因包含1683个核苷酸，与一株实验室株 Onderstepoort株的同源性为93.2%。推导的氨基酸序列包含523个氨基酸，与Onderstepoort株和一株强毒株A75/17株的氨基酸序列同源性分别为95.2%和99%。由于氨基酸序列的大多数差异发生在两个结构域，即N端和C端，我们进一步对其余3株野外分离株的3'端区域进行了测序。基于这些序列，新的CDV分离株有一个聚类，使它们与实验室株区分开来。
