白三烯对人乳腺癌细胞系MCF-7体外生长的影响。

Influence of leukotrienes on in vitro growth of human mammary carcinoma cell line MCF-7.

作者信息

Przylipiak A, Hafner J, Przylipiak J, Runnebaum B, Rabe T, Köhn F M

机构信息

Department of Obstetrics and Gynaecology, University of Heidelberg, Germany.

出版信息

Eur J Obstet Gynecol Reprod Biol. 1998 Mar;77(1):61-5.

PMID:9550202

Abstract

OBJECTIVES

The aim of this work was to study the action of leukotrienes on the growth of human mammary cancer cells MCF-7.

STUDY DESIGN

The growth of the cells was measured by incorporation of 3H-thymidine. The action of leukotriene (LT)B4, LTD4, LTC4, LTE4 or arachidonate (AA) was tested in human mammary cancer cells MCF-7 in vitro.

RESULTS

LTB4 or LTD4 but not LTC4 or LTE4 reduced significant incorporation of 3H-thymidine in MCF-7 cells up to 52% or 56% respectively, when administered in concentrations 0.1-1000 pM. Agents in concentrations of 0.01 pM or 10000 pM did not effect 3H-thymidine incorporation. We have shown, that MCF-7 cells synthesise LTB4 when treated with calcium ionophor A23187 (10 microM). Leukotriene-antagonist LY171883 (10 microM) lifts inhibitory effects of LTB4 or LTD4. Arachidonic acid (10 microM) inhibits 3H-thymidine incorporation up to 72%. 5-lipoxygenase inhibitor MK-886 (100 nM) lifts the inhibitory effect of arachidonate.

CONCLUSIONS

LTB4 or LTD4 inhibits MCF-7 breast cancer cell growth. LT-receptors mediate the growth-inhibitory effect of LTB4 or LTD4.

摘要

目的

本研究旨在探讨白三烯对人乳腺癌细胞MCF - 7生长的作用。

研究设计

通过掺入³H - 胸腺嘧啶核苷来测量细胞的生长。在体外对人乳腺癌细胞MCF - 7测试白三烯（LT）B4、LTD4、LTC4、LTE4或花生四烯酸（AA）的作用。

结果

当以0.1 - 1000 pM的浓度给药时，LTB4或LTD4可使MCF - 7细胞中³H - 胸腺嘧啶核苷的掺入量分别显著降低高达52%或56%，而LTC4或LTE4则无此作用。0.01 pM或10000 pM浓度的药物对³H - 胸腺嘧啶核苷的掺入无影响。我们发现，用钙离子载体A23187（10 μM）处理时，MCF - 7细胞可合成LTB4。白三烯拮抗剂LY171883（10 μM）可消除LTB4或LTD4的抑制作用。花生四烯酸（10 μM）可使³H - 胸腺嘧啶核苷的掺入量降低高达72%。5 - 脂氧合酶抑制剂MK - 886（100 nM）可消除花生四烯酸的抑制作用。