Fellowes R A, Maule A G, Marks N J, Geary T G, Thompson D P, Shaw C, Halton D W
Comparative Neuroendocrinology Research Group, The Schools of Biology and Biochemistry and Clinical Medicine, The Queen's University of Belfast, UK.
Parasitology. 1998 Mar;116 ( Pt 3):277-87. doi: 10.1017/s0031182097002229.
Ascaris suum contains a large number of FMRFamide-related peptides (FaRPs) of which KNEFIRFamide (AF1), KHEYLRFamide (AF2) and KSAYMRFamide (AF8, also called PF3) have been extensively studied and are known to exert actions on somatic muscle strips of the worm. In the present study, the effects of AF1, AF2 and AF8 on the activity of the vagina vera of female A. suum have been examined in vitro. The vagina vera is a muscular tube connecting the uterus and vagina uteri to the gonopore and is probably involved in regulating egg output. The tissue exhibited spontaneous, rhythmic contractions in vitro, which were modulated by each of the FaRPs tested. The effects of each of the peptides were qualitatively and quantitatively different, and in each case were reversible. AF1 (1 microM) caused a biphasic response in the form of a transient lengthening of the preparation, followed by a shortening; contractions were initially inhibited but resumed 5 min post-addition of the peptide. Lower concentrations (< or = 0.1 microM) induced a less marked effect, with rhythmic contractions returning 5 min post-addition. AF2 and AF8 reduced contraction frequency at concentrations > or = 0.1 microM. Both peptides also caused the tissue to shorten, although the effects of AF8 on baseline tension were inconsistent. The apparent potencies of AF1 and AF8 on contraction frequency of the vagina vera were 10-fold greater than AF2 and, unlike their actions on A. suum somatic body wall muscles, the actions of AF1 and AF2 were qualitatively different. Indeed, the effects of each of these FaRPs on the vagina vera were markedly different from those observed on the somatic muscle.
猪蛔虫含有大量FMRF酰胺相关肽（FaRPs），其中KNEFIRF酰胺（AF1）、KHEYLRF酰胺（AF2）和KSAYMRF酰胺（AF8，也称为PF3）已得到广泛研究，并且已知它们对该蠕虫的体肌条有作用。在本研究中，已在体外检测了AF1、AF2和AF8对雌性猪蛔虫真阴道活性的影响。真阴道是连接子宫和子宫阴道至生殖孔的肌性管道，可能参与调节产卵量。该组织在体外呈现自发的节律性收缩，这些收缩受到所测试的每种FaRPs的调节。每种肽的作用在定性和定量上均不同，并且在每种情况下都是可逆的。AF1（1微摩尔）引起双相反应，表现为制剂短暂延长，随后缩短；收缩最初受到抑制，但在添加该肽后5分钟恢复。较低浓度（≤0.1微摩尔）诱导的作用不太明显，添加后5分钟节律性收缩恢复。AF2和AF8在浓度≥0.1微摩尔时降低收缩频率。两种肽也导致组织缩短，尽管AF8对基线张力的影响不一致。AF1和AF8对真阴道收缩频率的表观效力比AF2高10倍，并且与它们对猪蛔虫体壁肌肉的作用不同，AF1和AF2的作用在定性上不同。实际上，这些FaRPs中的每一种对真阴道的作用与在体肌上观察到的作用明显不同。
