通过DNA异源双链分析进行MICA外显子5微卫星分型：跨膜区域的一种新多态性
MICA exon 5 microsatellite typing by DNA heteroduplex analysis: a new polymorphism in the transmembrane region.
作者信息
Vitiani L R, Potolicchio I, D'Amato M, Baricordi O R, Sorrentino R
机构信息
Dept. of Cell Biology and Development, University of Rome La Sapienza, Roma, Italy.
出版信息
Tissue Antigens. 1998 Mar;51(3):309-11. doi: 10.1111/j.1399-0039.1998.tb03108.x.
MICA (MHC class I chain-related gene A) is localized 47 kb upstream from HLA-B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.
MICA（主要组织相容性复合体I类链相关基因A）位于6号染色体短臂上HLA - B上游47 kb处。据推测，MICA蛋白的折叠方式与I类链相似，可能具有结合短配体的能力。该基因跨膜（TM）区域内的短串联重复序列（STR）已被描述，并且定义了由4至9个GCT密码子组成的五个等位基因，每个密码子编码一个丙氨酸残基。我们应用DNA异源双链分析对MICA三核苷酸重复序列进行分型，以开发一种简单可靠的鉴定方法。这种方法能够对所有MICA等位基因进行特征描述。此外，在TM区域内还鉴定出一种新的多态性。
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