Sechler J M, Yip J C, Rosenberg A S
Division of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
J Immunol. 1997 Dec 15;159(12):5766-8.
We designed a series of experiments to define the role of IFN-gamma in cellular interactions mediating graft rejection by assessing the rejection of H-Y disparate grafts in both ligand and receptor knockout mice and their control inbred strain. In the course of these studies it became apparent that neither knockout strain is histocompatible with the putative control and that the putative control is not histocompatible with either knockout strain. In the process of deducing why this might be so, it became apparent that the putative control is not an inbred strain of mouse. Thus, in the absence of rigorous genetic control, the utility of such knockout strains of mice for assessing the effects of cytokines and receptors in transplantation and autoimmunity is limited.
我们设计了一系列实验，通过评估H-Y不相容移植物在配体和受体基因敲除小鼠及其对照近交系中的排斥反应，来确定干扰素-γ在介导移植物排斥的细胞相互作用中的作用。在这些研究过程中，很明显，两种基因敲除品系与假定的对照都不存在组织相容性，并且假定的对照与任何一种基因敲除品系也不存在组织相容性。在推断为何会如此的过程中，很明显假定的对照不是小鼠的近交系。因此，在缺乏严格遗传控制的情况下，此类基因敲除小鼠品系用于评估细胞因子和受体在移植和自身免疫中的作用的效用是有限的。
