Arndt T, Hackler R, Kleine T O, Gressner A M
Abteilung für Klinische Chemie und Zentrallaboratorium, Philipps-Universität, Marburg, Germany.
Clin Chem. 1998 Jan;44(1):27-34.
Serum concentration of carbohydrate-deficient transferrin (CDT) is used for laboratory diagnosis of chronic alcohol abuse. Using isoelectric focusing for validation of the initial isotransferrin fractionation step involved in the determination of CDT by the CDTect assay, we found a complete in vitro iron saturation of transferrin and sufficient stability of the transferrin iron load during column passage; effective separation of non-CDT-isotransferrins and CDT-isotransferrins at the microcolumns; partial coelution of trisialo-Fe2-transferrin, which did not significantly affect CDT measurement; partial retention of CDT-isotransferrins, especially disialo-Fe2-transferrin, which may cause falsely negative results for CDT at the upper reference limits; good precision of the isotransferrin fractionation step; and no significant effects of low concentrations of serum protein and transferrin. We strongly urge standardization of CDT analysis and suggest isoelectric focusing for validation of CDT analysis methods and verification of odd results.
血清中缺糖转铁蛋白（CDT）的浓度用于慢性酒精滥用的实验室诊断。通过等电聚焦验证CDTect检测法测定CDT过程中初始异转铁蛋白分级分离步骤，我们发现转铁蛋白在体外完全被铁饱和，且在柱通过过程中转铁蛋白铁负载具有足够的稳定性；在微柱上能有效分离非CDT - 异转铁蛋白和CDT - 异转铁蛋白；三唾液酸 - Fe2 - 转铁蛋白部分共洗脱，这对CDT测量无显著影响；CDT - 异转铁蛋白存在部分保留，尤其是二唾液酸 - Fe2 - 转铁蛋白，这可能在参考上限导致CDT出现假阴性结果；异转铁蛋白分级分离步骤具有良好的精密度；低浓度血清蛋白和转铁蛋白无显著影响。我们强烈敦促CDT分析标准化，并建议采用等电聚焦来验证CDT分析方法及核实异常结果。
