冈田酸在DNA含量大于G2期的SV40感染的CV-1细胞中诱导有丝分裂表位MPM-2的出现。

Okadaic acid induces appearance of the mitotic epitope MPM-2 in SV40-infected CV-1 cells with a >G2-phase DNA content.

作者信息

Friedrich T D, Okubo E, Laffin J, Lehman J M

机构信息

Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, New York 12208, USA.

出版信息

Cytometry. 1998 Apr 1;31(4):260-4.

PMID:9551601

Abstract

Simian virus 40 (SV40) infection of quiescent monkey kidney cells stimulates two successive rounds of cellular DNA synthesis without an intervening mitosis. This uncoupling of S phase and mitosis indicates that SV40 modulates pathways regulating the G2-to-M phase transition. To examine the integrity of mitotic initiation pathways in infected cells that have bypassed mitosis, SV40-infected CV-1 cells were treated with okadaic acid (OA), a known inducer of premature mitosis in other cell types. OA treatment triggered the appearance of the mitotic marker MPM-2 in SV40-infected CV-1 cells progressing through either the first (diploid) or second (tetraploid) S phases. These results demonstrate that a subset of mitotic pathways are intact but inactive in SV40-infected cells that have bypassed mitosis and initiated tetraploid S phase.

摘要

猿猴病毒40（SV40）感染静止的猴肾细胞会刺激两轮连续的细胞DNA合成，且中间没有有丝分裂过程。S期和有丝分裂的这种解偶联表明SV40调节了调控G2期到M期转换的途径。为了检查绕过有丝分裂的感染细胞中有丝分裂起始途径的完整性，用冈田酸（OA）处理了被SV40感染的CV-1细胞，OA是其他细胞类型中已知的早熟有丝分裂诱导剂。OA处理在经历第一个（二倍体）或第二个（四倍体）S期的被SV40感染的CV-1细胞中触发了有丝分裂标记MPM-2的出现。这些结果表明，在绕过有丝分裂并启动四倍体S期的被SV40感染的细胞中，有丝分裂途径的一个子集是完整的但处于非活性状态。