Xu D, Leveugle B, Kreutz F T, Suresh M R
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.
J Chromatogr B Biomed Sci Appl. 1998 Mar 20;706(2):217-29. doi: 10.1016/s0378-4347(97)00441-6.
We developed a simple purification method to purify alkaline phosphatase/anti-alkaline phosphatase IgG as immune complexes using mimetic affinity chromatography wherein the antibody was either a monospecific antibody, a bispecific antibody or a commercial polyclonal IgG conjugated with alkaline phosphatase (AP-IgG) covalently. The immune complexes or conjugates were efficiently bound on the mimetic Blue A6XL column and eluted under mild conditions (5-20 mM phosphate buffer). A similar strategy of purifying peroxidase/anti-peroxidase antibody complexes was also successfully demonstrated using the mimetic Red 3 column. Mimetic affinity chromatography thus appears to be a simple method to purify the desired monospecific or bispecific antibodies from the respective hybridomas and quadromas.
我们开发了一种简单的纯化方法，利用模拟亲和色谱法将碱性磷酸酶/抗碱性磷酸酶IgG作为免疫复合物进行纯化，其中抗体可以是单特异性抗体、双特异性抗体或与碱性磷酸酶共价偶联的商业多克隆IgG（AP-IgG）。免疫复合物或偶联物能有效地结合在模拟蓝A6XL柱上，并在温和条件下（5 - 20 mM磷酸盐缓冲液）洗脱。使用模拟红3柱也成功证明了一种类似的纯化过氧化物酶/抗过氧化物酶抗体复合物的策略。因此，模拟亲和色谱法似乎是一种从相应的杂交瘤和四瘤中纯化所需单特异性或双特异性抗体的简单方法。
