Shan X, Bourdeau A, Rhoton A, Wells D E, Cohen E H, Landgraf B E, Palfree R G
McGill University, Royal Victoria Hospital, Montreal, Quebec, Canada.
J Immunol. 1998 Jan 1;160(1):197-208.
The 9804 gene, which encodes a human Ly-6 protein most similar to mouse differentiation Ag TSA-1/Sca-2, has also been called RIG-E. Like mouse TSA-1, it has a broad tissue distribution with varied expression levels in normal human tissues and tumor cell lines. Like some members of the murine Ly-6 family, the 9804 gene is responsive to IFNs, particularly IFN-alpha. Overlapping genomic fragments spanning the 9804 gene (5543 bp) have been isolated and characterized. The gene organization is analogous to that of known mouse Ly-6 genes. The first exon, 2296 bp upstream from exon II, is entirely untranslated. The three coding exons (II, III, and IV) are separated by short introns of 321 and 131 bp, respectively. Primers were developed for specific amplification of 9804 gene fragments. Screening of human-hamster somatic cell hybrids and yeast artificial chromosomes (YACs) indicated that the gene is distal to c-Myc, located in the q arm of human chromosome 8. No positives were detected from the Centre d'Etude du Polymorphisme Humain mega-YAC A or B panels, nor from bacterial artificial chromosome libraries; two positive cosmids (c101F1 and c157F6) were isolated from a human chromosome 8 cosmid library (LA08NC01). Fluorescence in situ hybridization of metaphase spreads of chromosome 8, containing hybrid cell line 706-B6 clone 17 (CL-17) with cosmid c101F1, placed the 9804 gene close to the telomere at 8q24.3. This mapping is significant, since the region shares a homology with a portion of mouse chromosome 15, which extends into band E where Ly-6 genes reside. Moreover, the gene encoding E48, the homologue of mouse Ly-6 molecule ThB, has also been mapped to 8q24.
9804基因编码一种与小鼠分化抗原TSA-1/Sca-2最为相似的人类Ly-6蛋白，也被称为RIG-E。与小鼠TSA-1一样，它在正常人体组织和肿瘤细胞系中具有广泛的组织分布，表达水平各不相同。与鼠Ly-6家族的一些成员一样，9804基因对干扰素，尤其是干扰素-α有反应。跨越9804基因（5543 bp）的重叠基因组片段已被分离和鉴定。该基因的组织结构与已知的小鼠Ly-6基因类似。第一个外显子位于外显子II上游2296 bp处，完全不编码。三个编码外显子（II、III和IV）分别被321 bp和131 bp的短内含子隔开。已开发出用于特异性扩增9804基因片段的引物。对人-仓鼠体细胞杂种和酵母人工染色体（YAC）的筛选表明，该基因位于c-Myc远端，位于人类染色体8的q臂上。在人类多态性研究中心的巨型YAC A或B板以及细菌人工染色体文库中均未检测到阳性信号；从人类染色体8粘粒文库（LA08NC01）中分离出两个阳性粘粒（c101F1和c157F6）。用粘粒c101F1对含有杂交细胞系706-B6克隆17（CL-17）的8号染色体中期铺展进行荧光原位杂交，将9804基因定位在8q24.3靠近端粒的位置。这一图谱具有重要意义，因为该区域与小鼠染色体15的一部分具有同源性，该部分延伸到Ly-6基因所在的E带。此外，编码小鼠Ly-6分子ThB同源物E48的基因也已定位到8q24。
