出生后早期婴儿大肠中的T细胞与HLA-II类分子表达

T-cells and HLA-class II expression in the large intestine of infants in the early postnatal period.

作者信息

Ormälä T, Rintala R, Savilahti E

机构信息

Department of Pediatrics, Kuopio University Hospital, Finland.

出版信息

J Pediatr Gastroenterol Nutr. 1998 Apr;26(4):422-8. doi: 10.1097/00005176-199804000-00011.

DOI:10.1097/00005176-199804000-00011

PMID:9552138

Abstract

BACKGROUND

There is only limited knowledge of the development of the immune responses of the gut in very young infants after exposure to bacterial and food antigens at birth.

METHODS

In this study, 49 large intestinal biopsy specimens, which were judged to have normal morphology, were taken from 49 young infants. Eleven patients had Hirschsprung's disease (group 1) and 38 had miscellaneous conditions (group 2). The densities of T cells, their subsets expressing surface antigens CD8 and CD4, and T-cell receptors alpha/beta or gamma/beta were measured, as well as densities of mononuclear and epithelial cells expressing HLA-class II antigens.

RESULTS

T-cell densities in groups 1 and 2 were similar. Patients with Hirschsprung's disease had significantly more HLA-DR (p = 0.006) and HLA-DP-expressing cells (p = 0.003) in the lamina propria than did the patients in group 2. In group 1, HLA-DR- (r = 0.58; p = 0.46) and HLA-DP-expressing cells (r = 0.66; p = 0.03) showed a significant positive regression with age in the lamina propria, whereas in group 2, HLA-DR+ cells in the lamina propria showed marked (r = -0.9; p = 0.006) negative regression during the first 1.5 months of life. In contrast to results in previous reports, in the current results, HLA-D region antigens were present in the epithelium in a considerable proportion (up to one fourth) of specimens from the large intestine in both groups. CD3+ (r = -0.59; p = 0.006) and CD4+ (r = -0.64; p = 0.002) cells showed a strong negative regression with age in the lamina propria during the first 2.5 months; and thereafter, there was a weak, insignificant rise in the numbers of these cells. The distribution of CD4+, CD8+, and TCR alpha/beta or gamma/beta T cells of the epithelium of the young infants did not differ significantly from that in the epithelium of adults.

CONCLUSIONS

These results show that several significant changes occur in the mucosal immune system during the first few weeks of life.

摘要

背景

对于极年幼婴儿出生后接触细菌和食物抗原后肠道免疫反应的发育情况，人们了解有限。

方法

在本研究中，从49名幼儿身上获取了49份形态学判定为正常的大肠活检标本。11名患者患有先天性巨结肠症（第1组），38名患有其他病症（第2组）。测量了T细胞密度、表达表面抗原CD8和CD4的T细胞亚群密度以及T细胞受体α/β或γ/β密度，还测量了表达HLA - II类抗原的单核细胞和上皮细胞密度。

结果

第1组和第2组的T细胞密度相似。先天性巨结肠症患者固有层中表达HLA - DR（p = 0.006）和HLA - DP的细胞显著多于第2组患者。在第1组中，固有层中表达HLA - DR（r = 0.58；p = 0.46）和HLA - DP的细胞（r = 0.66；p = 0.03）与年龄呈显著正相关，而在第2组中，固有层中HLA - DR⁺细胞在出生后的前1.5个月呈显著（r = -0.9；p = 0.006）负相关。与先前报告的结果相反，在当前结果中，两组大肠标本中相当比例（高达四分之一）的上皮细胞中存在HLA - D区域抗原。在出生后的前2.5个月，固有层中CD3⁺（r = -0.59；p = 0.006）和CD4⁺（r = -0.64；p = 0.002）细胞与年龄呈强烈负相关；此后，这些细胞数量有微弱的、不显著的增加。幼儿上皮细胞中CD⁴⁺、CD⁸⁺以及TCRα/β或γ/β T细胞的分布与成人上皮细胞中的分布无显著差异。