猪甲状腺细胞质膜的化学成分。

Chemical composition of porcine thyroid cell plasma membranes.

作者信息

Verrier B, Planells R, Hennen G, Lissitzky S

出版信息

Mol Cell Endocrinol. 1976 Aug-Sep;5(3-4):215-21. doi: 10.1016/0303-7207(76)90085-x.

Abstract

Plasma membranes were isolated from thyroid cells obtained by trypsinization of porcine glands and maintained in culture conditions in the presence or absence of thyrotropin or dibutyryl cyclic AMP. The protein, phospholipid, cholesterol and sialic acid content of the 3 types of cell plasma membranes were very similar. High cholesterol and sialic acid content characterized these membranes. The amino acid and carbohydrate composition was similar to that shown for other eukaryotic plasma membranes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis disclosed the presence of more than 20 protein bands, of which six corresponded to glycoproteins.

摘要

通过对猪甲状腺进行胰蛋白酶消化获得甲状腺细胞，分离出质膜，并在有或无促甲状腺激素或二丁酰环磷酸腺苷的培养条件下进行培养。三种类型的细胞质膜的蛋白质、磷脂、胆固醇和唾液酸含量非常相似。这些膜的特征是胆固醇和唾液酸含量高。氨基酸和碳水化合物组成与其他真核细胞质膜的组成相似。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示存在20多条蛋白带，其中六条对应糖蛋白。