Watson D L
Immunology. 1976 Aug;31(2):159-65.
Non-lactating, multiparous ewes were immunized either by subcutaneous infection with live Staphylococcus aureus (seventeen ewes) or by intramuscular injections of a killed S. aureus-oil adjuvant vaccine (seventeen ewes). Polymorphs which were subsequently collected from the mammary glands of the animals were used in in vitro phagocytosis assays against Pseudomonas sp. or S. aureus. There was no difference between polymorphs from the two groups of ewes in their ability to phagocytose Pseudomonas organisms. Polymorphs from the infected ewes showed significant phagocytic superiority over cells from ewes given the killed vaccine when S. aureus was the target organism. This phagocytic superiority could be abrogated by removal of cytophilic immunoglobulin from polymorphs and restored by replacement of cytophilic immunoglobulin. It was shown by staining polymorphs with FITC-conjugated anti-immunoglobulin sera that cytophilic immunoglobulin on the surface of polymorphs belonged to the IgG2 class of immunoglobulins. When 'neutral' polymorphs (from non-immunized ewes) were coated with IgG2 purified from the sera of infected ewes, they exhibited enhanced phagocytosis of staphylococci compared with 'neutral' polymorphs carying IgG2 from the sera of ewes given the killed vaccine.
未产奶的经产母羊被分为两组进行免疫，一组通过皮下注射活的金黄色葡萄球菌进行免疫（17只母羊），另一组通过肌肉注射灭活的金黄色葡萄球菌-油佐剂疫苗进行免疫（17只母羊）。随后从这些动物乳腺中收集的多形核白细胞被用于针对假单胞菌属或金黄色葡萄球菌的体外吞噬试验。两组母羊的多形核白细胞在吞噬假单胞菌属微生物的能力上没有差异。当金黄色葡萄球菌作为靶标微生物时，来自感染母羊的多形核白细胞比接种灭活疫苗母羊的细胞表现出显著的吞噬优势。去除多形核白细胞上的亲细胞免疫球蛋白可消除这种吞噬优势，而重新添加亲细胞免疫球蛋白则可恢复该优势。用异硫氰酸荧光素（FITC）偶联的抗免疫球蛋白血清对多形核白细胞进行染色显示，多形核白细胞表面的亲细胞免疫球蛋白属于免疫球蛋白IgG2类。当用从感染母羊血清中纯化的IgG2包被“中性”多形核白细胞（来自未免疫母羊）时，与携带接种灭活疫苗母羊血清中IgG2的“中性”多形核白细胞相比，它们对葡萄球菌的吞噬作用增强。
