Kerlin R L, Watson D L
Res Vet Sci. 1986 Sep;41(2):191-5.
Experiments in sheep were carried out to examine factors modifying the immunoglobulin (Ig) isotype of the antibody response to Brucella abortus. Live B abortus (S19) stimulated higher titres of agglutinating antibody and IgG1 and IgG2 antibody than did killed B abortus. Live B abortus stimulated a more protracted synthesis of IgG2 antibody during the primary and secondary responses than did the killed S19 vaccine. In a second experiment, the capacity of live and killed Staphylococcus aureus to modify the antibody response to killed B abortus was examined. Both live and killed S aureus enhanced production of anti-brucella antibodies; this response was attributed to the adjuvant properties of S aureus. Killed S aureus enhanced production of anti-brucella antibody to a greater extent than live S aureus. Live S aureus did not preferentially enhance production of IgG2 anti-brucella antibody. The results suggested that the enhanced production of IgG2 antibody induced by live vaccines does not depend solely on a pyogenic lesion at the vaccination site.
开展了绵羊实验，以研究影响针对流产布鲁氏菌抗体应答免疫球蛋白（Ig）同种型的因素。与灭活的流产布鲁氏菌相比，活的流产布鲁氏菌（S19）刺激产生的凝集抗体以及IgG1和IgG2抗体滴度更高。在初次和二次应答期间，活的流产布鲁氏菌刺激产生IgG2抗体的时间比灭活的S19疫苗更长。在第二个实验中，研究了活的和灭活的金黄色葡萄球菌改变针对灭活流产布鲁氏菌抗体应答的能力。活的和灭活的金黄色葡萄球菌均增强了抗布鲁氏菌抗体的产生；这种应答归因于金黄色葡萄球菌的佐剂特性。灭活的金黄色葡萄球菌比活的金黄色葡萄球菌更能增强抗布鲁氏菌抗体的产生。活的金黄色葡萄球菌不会优先增强IgG2抗布鲁氏菌抗体的产生。结果表明，活疫苗诱导的IgG2抗体产生增加并非仅取决于接种部位的化脓性病变。
