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哺乳期和断奶期母鼠乳腺组织、肝脏和骨骼肌中的亮氨酸分解代谢。

Leucine catabolism in mammary tissue, liver and skeletal muscle of dam rat during lactation and weaning.

作者信息

DeSantiago S, Torres N, Tovar A R

机构信息

Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F., México.

出版信息

Arch Med Res. 1998 Spring;29(1):25-32.

PMID:9556919
Abstract

BACKGROUND

This study was designed to determine the effect of lactation and weaning on the catabolism of branched-chain amino acids (BCAA).

METHODS

Rates of transamination and oxidation of leucine and branched chain alpha-ketoacid dehydrogenase (BCKD) activity were measured in homogenates of mammary gland, skeletal muscle and liver on day 12 of lactation and 24 h after separation of dams from the litter (weaning).

RESULTS

Lactating dams consumed 250% more protein than control rats, extra protein is required for protein synthesis by the mammary gland, the extent to which the excess of amino acids consumed during lactation is utilized or oxidized by different tissues is not known. The rate of transamination of [1-14C] leucine by mammary tissue of lactating dams was sixfold higher than in virgin rats. The rate of transamination remained elevated fourfold in postweaning dams. Rates of transamination were three times higher in mammary tissue than in muscle of lactating dams. Rate of oxidation [1-14C] leucine by lactating mammary tissue was tenfold higher than in control tissue.

CONCLUSIONS

The capacity of mammary tissue for transamination and oxidation of leucine increased greatly during lactation, suggesting that the mammary gland may play an important role in the catabolism of BCAA during lactation.

摘要

背景

本研究旨在确定泌乳和断奶对支链氨基酸(BCAA)分解代谢的影响。

方法

在泌乳第12天以及母鼠与幼崽分离24小时后(断奶),测定乳腺、骨骼肌和肝脏匀浆中亮氨酸的转氨和氧化速率以及支链α-酮酸脱氢酶(BCKD)活性。

结果

泌乳母鼠摄入的蛋白质比对照大鼠多250%,乳腺蛋白质合成需要额外的蛋白质,泌乳期间摄入的过量氨基酸被不同组织利用或氧化的程度尚不清楚。泌乳母鼠乳腺组织中[1-14C]亮氨酸的转氨速率比未生育大鼠高6倍。断奶后母鼠的转氨速率仍比未生育大鼠高4倍。泌乳母鼠乳腺组织中的转氨速率比肌肉中的高3倍。泌乳乳腺组织中[1-14C]亮氨酸的氧化速率比对照组织高10倍。

结论

泌乳期间乳腺组织中亮氨酸的转氨和氧化能力大幅增加,表明乳腺在泌乳期间BCAA的分解代谢中可能起重要作用。

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