人类免疫缺陷病毒1型核衣壳蛋白N端锌指在病毒结构与复制中的作用
Role of the N-terminal zinc finger of human immunodeficiency virus type 1 nucleocapsid protein in virus structure and replication.
作者信息
Tanchou V, Decimo D, Péchoux C, Lener D, Rogemond V, Berthoux L, Ottmann M, Darlix J L
机构信息
LaboRetro, Unité de Virologie Humaine INSERM U412, Ecole Normale Supérieure de Lyon, France.
出版信息
J Virol. 1998 May;72(5):4442-7. doi: 10.1128/JVI.72.5.4442-4447.1998.
Abstract
Nucleocapsid protein (NCp7) of human immunodeficiency virus type 1 is found covering the genomic RNA in the interior of the viral particle. It is a highly basic protein with two zinc fingers of the form CX2CX4HX4C which exhibit strong affinity for a zinc cation. To study the structure-function relationship of the N-terminal zinc finger of NCp7, this domain was either deleted or changed to CX2CX4CX4C. We examined virus formation and structure as well as proviral DNA synthesis. Our data show that these two NC mutations result in the formation of particles with an abnormal core morphology and impair the end of proviral DNA synthesis, leading to noninfectious viruses.
摘要
1型人类免疫缺陷病毒的核衣壳蛋白（NCp7）被发现覆盖在病毒颗粒内部的基因组RNA上。它是一种高度碱性的蛋白质，具有两个CX2CX4HX4C形式的锌指结构，对锌阳离子表现出很强的亲和力。为了研究NCp7 N端锌指的结构-功能关系，该结构域要么被删除，要么被改变为CX2CX4CX4C。我们检查了病毒的形成、结构以及前病毒DNA的合成。我们的数据表明，这两种NC突变导致形成具有异常核心形态的颗粒，并损害前病毒DNA合成的末端，从而产生无感染性的病毒。
相似文献
1
Role of the N-terminal zinc finger of human immunodeficiency virus type 1 nucleocapsid protein in virus structure and replication.
J Virol. 1998 May;72(5):4442-7. doi: 10.1128/JVI.72.5.4442-4447.1998.
2
Mutations in the N-terminal domain of human immunodeficiency virus type 1 nucleocapsid protein affect virion core structure and proviral DNA synthesis.
J Virol. 1997 Sep;71(9):6973-81. doi: 10.1128/JVI.71.9.6973-6981.1997.
3
1H NMR structure and biological studies of the His23-->Cys mutant nucleocapsid protein of HIV-1 indicate that the conformation of the first zinc finger is critical for virus infectivity.
Biochemistry. 1994 Oct 4;33(39):11707-16. doi: 10.1021/bi00205a006.
4
The central globular domain of the nucleocapsid protein of human immunodeficiency virus type 1 is critical for virion structure and infectivity.
J Virol. 1995 Mar;69(3):1778-84. doi: 10.1128/JVI.69.3.1778-1784.1995.
5
Effect of mutations in the nucleocapsid protein (NCp7) upon Pr160(gag-pol) and tRNA(Lys) incorporation into human immunodeficiency virus type 1.
J Virol. 1997 Jun;71(6):4378-84. doi: 10.1128/JVI.71.6.4378-4384.1997.
6
Interactions between HIV-1 nucleocapsid protein and viral DNA may have important functions in the viral life cycle.
Nucleic Acids Res. 1993 Feb 25;21(4):831-9. doi: 10.1093/nar/21.4.831.
7
Nucleocapsid and matrix protein contributions to selective human immunodeficiency virus type 1 genomic RNA packaging.
J Virol. 1998 Mar;72(3):1983-93. doi: 10.1128/JVI.72.3.1983-1993.1998.
8
The role of nucleocapsid of HIV-1 in virus assembly.
Virology. 1998 Nov 10;251(1):141-57. doi: 10.1006/viro.1998.9374.
9
Multiple effects of an anti-human immunodeficiency virus nucleocapsid inhibitor on virus morphology and replication.
J Virol. 1999 Dec;73(12):10000-9. doi: 10.1128/JVI.73.12.10000-10009.1999.
10
Mutational analysis of two zinc finger motifs in HIV type 1 nucleocapsid proteins: effects on proteolytic processing of Gag precursors and particle formation.
AIDS Res Hum Retroviruses. 1996 Jun 10;12(9):793-800. doi: 10.1089/aid.1996.12.793.
引用本文的文献
1
Influence of HIV-1 Genomic RNA on the Formation of Gag Biomolecular Condensates.
J Mol Biol. 2023 Aug 15;435(16):168190. doi: 10.1016/j.jmb.2023.168190. Epub 2023 Jun 27.
2
Crucial mutation in the exoribonuclease domain of nsp14 of PEDV leads to high genetic instability during viral replication.
Cell Biosci. 2021 Jun 7;11(1):106. doi: 10.1186/s13578-021-00598-1.
3
Overview of the Nucleic-Acid Binding Properties of the HIV-1 Nucleocapsid Protein in Its Different Maturation States.
Viruses. 2020 Sep 29;12(10):1109. doi: 10.3390/v12101109.
4
Nucleocapsid Protein Precursors NCp9 and NCp15 Suppress ATP-Mediated Rescue of AZT-Terminated Primers by HIV-1 Reverse Transcriptase.
Antimicrob Agents Chemother. 2020 Sep 21;64(10). doi: 10.1128/AAC.00958-20.
5
Zinc Fingers in HIV-1 Gag Precursor Are Not Equivalent for gRNA Recruitment at the Plasma Membrane.
Biophys J. 2020 Jul 21;119(2):419-433. doi: 10.1016/j.bpj.2020.05.035. Epub 2020 Jun 12.
6
RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane.
Viruses. 2020 Apr 15;12(4):447. doi: 10.3390/v12040447.
7
Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking.
Cell Rep. 2020 Apr 21;31(3):107520. doi: 10.1016/j.celrep.2020.03.084.
8
Effect of Human Immunodeficiency Virus on Trace Elements in the Brain.
Biol Trace Elem Res. 2021 Jan;199(1):41-52. doi: 10.1007/s12011-020-02129-4. Epub 2020 Apr 1.
9
Zinc, copper, CD4 T-cell count and some hematological parameters of HIV-infected subjects in Southern Nigeria.
Integr Med Res. 2018 Mar;7(1):53-60. doi: 10.1016/j.imr.2018.01.008. Epub 2018 Feb 13.
10
Cross- and Co-Packaging of Retroviral RNAs and Their Consequences.
Viruses. 2016 Oct 11;8(10):276. doi: 10.3390/v8100276.
本文引用的文献
1
Mutations in the N-terminal domain of human immunodeficiency virus type 1 nucleocapsid protein affect virion core structure and proviral DNA synthesis.
J Virol. 1997 Sep;71(9):6973-81. doi: 10.1128/JVI.71.9.6973-6981.1997.
2
Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitro.
J Virol. 1997 Aug;71(8):6225-9. doi: 10.1128/JVI.71.8.6225-6229.1997.
3
Human immunodeficiency virus type 1 nucleocapsid protein promotes efficient strand transfer and specific viral DNA synthesis by inhibiting TAR-dependent self-priming from minus-strand strong-stop DNA.
J Virol. 1997 Jul;71(7):5178-88. doi: 10.1128/JVI.71.7.5178-5188.1997.
4
Possible roles of HIV-1 nucleocapsid protein in the specificity of proviral DNA synthesis and in its variability.
J Mol Biol. 1997 May 2;268(2):250-60. doi: 10.1006/jmbi.1997.0978.
5
Interaction of retroviral nucleocapsid proteins with transfer RNAPhe: a lead ribozyme and 1H NMR study.
Nucleic Acids Res. 1996 Sep 15;24(18):3568-75. doi: 10.1093/nar/24.18.3568.
6
Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract.
J Virol. 1996 Oct;70(10):7132-42. doi: 10.1128/JVI.70.10.7132-7142.1996.
7
Charged amino acid residues of human immunodeficiency virus type 1 nucleocapsid p7 protein involved in RNA packaging and infectivity.
J Virol. 1996 Oct;70(10):6607-16. doi: 10.1128/JVI.70.10.6607-6616.1996.
8
Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site.
J Virol. 1996 Aug;70(8):4996-5004. doi: 10.1128/JVI.70.8.4996-5004.1996.
9
HIV-1 nucleocapsid protein induces "maturation" of dimeric retroviral RNA in vitro.
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7577-81. doi: 10.1073/pnas.93.15.7577.
10
Mutational analysis of two zinc finger motifs in HIV type 1 nucleocapsid proteins: effects on proteolytic processing of Gag precursors and particle formation.
AIDS Res Hum Retroviruses. 1996 Jun 10;12(9):793-800. doi: 10.1089/aid.1996.12.793.
Zotero 插件