携带一组来自冷传代呼吸道合胞病毒（RSV）突变的重组RSV在黑猩猩中减毒。
Recombinant respiratory syncytial virus (RSV) bearing a set of mutations from cold-passaged RSV is attenuated in chimpanzees.
作者信息
Whitehead S S, Juhasz K, Firestone C Y, Collins P L, Murphy B R
机构信息
Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0720, USA.
出版信息
J Virol. 1998 May;72(5):4467-71. doi: 10.1128/JVI.72.5.4467-4471.1998.
Abstract
A set of five missense mutations previously identified by nucleotide sequence analysis of subgroup A cold-passaged (cp) respiratory syncytial virus (RSV) has been introduced into a recombinant wild-type strain of RSV. This recombinant virus, designated rA2cp, appears to replicate less efficiently in the upper and lower respiratory tracts of seronegative chimpanzees than either biologically derived or recombinant wild-type RSV. Infection with rA2cp also resulted in significantly less rhinorrhea and cough than infection with wild-type RSV. These findings confirm the role of the cp mutations in attenuation of RSV and identify their usefulness for inclusion in future live attenuated recombinant RSV vaccine candidates.
摘要
通过对A亚组冷传代（cp）呼吸道合胞病毒（RSV）进行核苷酸序列分析先前鉴定出的一组五个错义突变已被引入到RSV的重组野生型毒株中。这种重组病毒，命名为rA2cp，在血清阴性黑猩猩的上、下呼吸道中似乎比生物来源的或重组野生型RSV复制效率更低。与野生型RSV感染相比，rA2cp感染导致的鼻漏和咳嗽也明显更少。这些发现证实了cp突变在RSV减毒中的作用，并确定了它们在未来减毒活重组RSV疫苗候选株中的应用价值。
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