Barwicz J, Dumont I, Ouellet C, Gruda I
Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Canada.
Biospectroscopy. 1998;4(2):135-44. doi: 10.1002/(sici)1520-6343(1998)4:2<135::aid-bspy6>3.0.co;2-4.
The effect of amphotericin B on the oxidation and degradation of low- and high-density lipoproteins was investigated by UV-vis spectroscopy, electron microscopy, electrophoresis, and size-exclusion chromatography. Two formulations of the drug were used: the commercial Fungizone and a new, less toxic, liposomal formulation, AmBisome. It was shown that Fungizone strongly enhanced the oxidative deformation of low-density lipoprotein structure while AmBisome did not bind to this lipoprotein fraction and did not affect its oxidation. It was shown that amphotericin B contained in Fungizone extracted cholesterol from low-density lipoproteins which sensitized them to oxidation. Both formulations of amphotericin B studied here did not bind to high-density lipoprotein and did not affect the process of its oxidation.
通过紫外可见光谱、电子显微镜、电泳和尺寸排阻色谱法研究了两性霉素B对低密度脂蛋白和高密度脂蛋白氧化及降解的影响。使用了该药物的两种制剂：市售的两性霉素B（商品名：Fungizone）和一种新的、毒性较小的脂质体制剂安必素（AmBisome）。结果表明，Fungizone强烈增强了低密度脂蛋白结构的氧化变形，而安必素不与该脂蛋白组分结合，也不影响其氧化。结果表明，Fungizone中含有的两性霉素B从低密度脂蛋白中提取胆固醇，使其对氧化敏感。本文研究的两种两性霉素B制剂均不与高密度脂蛋白结合，也不影响其氧化过程。
