Kannari N, Ohshima H, Maeda T, Noda T, Takano Y
Department of Pediatric Dentistry, Niigata University School of Dentistry, Japan.
Arch Histol Cytol. 1998 Mar;61(1):1-15. doi: 10.1679/aohc.61.1.
The distribution and ultrastructure of the class II major histocompatibility complex (MHC) antigen-expressing cells in the pulp tissue of human deciduous teeth during the process of physiological root resorption was surveyed by histochemical and immunocytochemical methods using an anti-human leukocyte antigen (HLA)-DR-monoclonal antibody. Dental pulp was found to contain numerous HLA-DR-positive cells of various shapes; those showing a dendritic appearance were located mainly in the periphery of the pulp tissue, associated closely with the odontoblasts. The immunopositive cells sometimes extended their cytoplasmic processes into the dentinal tubules and increased in number in the areas affected by dental caries, attrition or restorative procedures, implicating their role in immunosurveillance. The immunopositive cells were located consistently at the pulp-dentin border during the stage of active resorption, adjacent to the preodontoclasts or odontoclasts, and covered the exposed dentin surface after the detachment of the odontoclasts until the onset of cementum formation. These data suggest that the HLA-DR-immunopositive cells in the coronal pulp of human deciduous teeth play an inductive role in the differentiation, migration and/or activation of the odontoclasts and cementoblast-like cells during the stages of tooth resorption.
采用抗人白细胞抗原（HLA）-DR单克隆抗体，通过组织化学和免疫细胞化学方法，研究了生理性牙根吸收过程中人类乳牙牙髓组织中表达Ⅱ类主要组织相容性复合体（MHC）抗原的细胞的分布及超微结构。发现牙髓中含有许多形态各异的HLA-DR阳性细胞；呈树突状外观的细胞主要位于牙髓组织周边，与成牙本质细胞紧密相连。免疫阳性细胞有时将其细胞质突起延伸至牙本质小管，且在受龋齿、磨损或修复操作影响的区域数量增加，提示它们在免疫监视中发挥作用。在活跃吸收阶段，免疫阳性细胞始终位于牙髓-牙本质交界处，紧邻前破牙细胞或破牙细胞，在破牙细胞脱离后覆盖暴露的牙本质表面，直至牙骨质形成开始。这些数据表明，人类乳牙冠髓中的HLA-DR免疫阳性细胞在牙齿吸收阶段破牙细胞和成牙骨质样细胞的分化、迁移和/或激活中起诱导作用。
