通过注射MRL/lpr小鼠脾细胞在SCID小鼠中诱导免疫介导的听力损失。

Induction of immune-mediated hearing loss in SCID mice by injection of MRL/lpr mouse spleen cells.

作者信息

Iwai H, Tomoda K, Hosaka N, Miyashima S, Suzuka Y, Ikeda H, Lee S, Inaba M, Ikehara S, Yamashita T

机构信息

Department of Otorhinolaryngology, Kansai Medical University, Moriguchi, Osaka, Japan.

出版信息

Hear Res. 1998 Mar;117(1-2):173-7. doi: 10.1016/s0378-5955(98)00015-x.

DOI:10.1016/s0378-5955(98)00015-x

PMID:9557987

Abstract

Induction of immune-mediated hearing loss in SCID mice by injection of MRL/lpr mouse spleen cells The MRL/lpr mouse, which has a mutation in the Fas gene encoding a cell-surface receptor for apoptosis, shows an accumulation of abnormal immunocompetent cells and SLE-like disease. It has recently been reported that this mouse also manifests sensorineural hearing loss (SHL) with cochlear pathology at 20 weeks of age. We examined the effects of injecting MRL/lpr spleen cells on the development of SHL in severe combined immunodeficient (SCID) mice, which originally develop neither SHL nor cochlear pathology. Immune-mediated SHL and cochlear pathology were, indeed, transferred to the SCID mice by the injection of spleen cells from the MRL/lpr mice. These findings suggest that cell-mediated immunity is involved in the development of SHL and cochlear pathology.

摘要

通过注射MRL/lpr小鼠脾细胞诱导SCID小鼠发生免疫介导性听力损失

Fas基因（编码一种细胞表面凋亡受体）发生突变的MRL/lpr小鼠，表现出异常免疫活性细胞的积累和类似系统性红斑狼疮的疾病。最近有报道称，这种小鼠在20周龄时还会出现感音神经性听力损失（SHL）并伴有耳蜗病理改变。我们研究了注射MRL/lpr脾细胞对严重联合免疫缺陷（SCID）小鼠SHL发生发展的影响，SCID小鼠原本既不发生SHL也无耳蜗病理改变。实际上，通过注射来自MRL/lpr小鼠的脾细胞，免疫介导的SHL和耳蜗病理改变被转移到了SCID小鼠身上。这些发现表明，细胞介导的免疫反应参与了SHL和耳蜗病理改变的发生发展。