Madara J L
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Annu Rev Physiol. 1998;60:143-59. doi: 10.1146/annurev.physiol.60.1.143.
The intercellular tight junction is the rate-limiting barrier in the paracellular pathway for permeation by ions and larger solutes. A variety of widely used electrical and flux approaches are used in the analyses of solute permeation through this pathway; however, each has limitations in practice. It is now clear that solute permeation across tight junctions is dynamically regulated by intracellular events with a common effector mechanism apparently tied to the cytoskeleton. These pathways, which regulate tight junction solute permeability, are targets that produce epithelial barrier dysfunction in a variety of disease states. However, regulation of solute permeation across the junctional barrier may also represent a potential means to improve bioavailability of orally administered bioactive solutes.
细胞间紧密连接是离子和较大溶质通过细胞旁途径渗透的限速屏障。在分析溶质通过该途径的渗透时，使用了多种广泛应用的电学和通量方法；然而，每种方法在实际应用中都有局限性。现在已经清楚，溶质通过紧密连接的渗透受到细胞内事件的动态调节，一种常见的效应机制显然与细胞骨架相关。这些调节紧密连接溶质通透性的途径是在多种疾病状态下导致上皮屏障功能障碍的靶点。然而，调节溶质通过连接屏障的渗透也可能是提高口服生物活性溶质生物利用度的一种潜在手段。
