Schwartz E, Sapadin A N, Kligman L H
Department of Dermatology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Arch Dermatol Res. 1998 Mar;290(3):137-44. doi: 10.1007/s004030050279.
Chronically sun-damaged human skin has a wrinkled, aged appearance as a result of alterations in the dermal extracellular matrix. Secondary effectors such as cytokines and integrins may mediate the effects of UV radiation on the skin by regulating the synthesis of metalloproteinases and structural proteins including collagen. The aim of this study was to semi-quantify the steady-state mRNA levels of interleukin-1 alpha, tumor necrosis factor alpha, transforming growth factor beta, collagenase, stromelysin, collagen, and integrins (alpha, and alpha2) in the skin of hairless mice that were either treated with UV or concurrently treated with UV and topical tretinoin for 5 weeks. Total RNA was extracted from the skin of the mice, reverse transcribed to cDNA, and amplified by the polymerase chain reaction in the presence of 32P-dCTP using gene-specific primers. Results were normalized relative to glyceraldehyde-3-phosphate dehydrogenase levels. Steady-state mRNA levels of the cytokines and integrins were increased by UV radiation. Concurrent UV and topical tretinoin treatment superinduced the expression of interleukin-1, increased alpha 1, and decreased alpha 2 integrin expression. Immunofluorescence analysis showed increased dermal localization of beta 1 integrin in UV and tretinoin treated skin. These results suggest that cytokines and integrins may be involved in the mechanism of photo-damage.
由于真皮细胞外基质的改变，长期受阳光损伤的人类皮肤呈现出皱纹、老化的外观。细胞因子和整合素等二级效应分子可能通过调节金属蛋白酶和包括胶原蛋白在内的结构蛋白的合成来介导紫外线辐射对皮肤的影响。本研究的目的是对无毛小鼠皮肤中白细胞介素-1α、肿瘤坏死因子α、转化生长因子β、胶原酶、基质溶解素、胶原蛋白和整合素（α1和α2）的稳态mRNA水平进行半定量分析，这些小鼠分别接受紫外线照射或同时接受紫外线照射和外用维甲酸治疗5周。从小鼠皮肤中提取总RNA，反转录为cDNA，并使用基因特异性引物在32P-dCTP存在的情况下通过聚合酶链反应进行扩增。结果相对于甘油醛-3-磷酸脱氢酶水平进行标准化。紫外线辐射增加了细胞因子和整合素的稳态mRNA水平。紫外线和外用维甲酸联合治疗超诱导白细胞介素-1的表达，增加α1整合素表达并降低α2整合素表达。免疫荧光分析显示，在紫外线和维甲酸处理的皮肤中，β1整合素在真皮中的定位增加。这些结果表明，细胞因子和整合素可能参与了光损伤机制。
