Dhaliwal D K, Barnhorst D A, Romanowski E, Rehkopf P G, Gordon Y J
Department of Ophthalmology, University of Pittsburgh Medical Center, Pennsylvania, USA.
Am J Ophthalmol. 1998 Apr;125(4):488-92. doi: 10.1016/s0002-9394(99)80189-5.
To investigate the role of excimer laser keratectomy as a trigger for the reactivation of latent HSV type 1 (HSV-1) in the New Zealand rabbit ocular model. There are conflicting reports in the current literature about reactivation of HSV-1 after excimer laser photoablation.
New Zealand rabbits were inoculated topically with HSV-1 McKrae or W strain in each eye, and culture-positive dendritic keratitis was documented on day 7. After the establishment of latency (21+ days), animals were divided into three groups: group I animals underwent excimer laser photoablation in each eye; group II animals received intrastromal injections of sterile water to act as positive controls (a standard method); and group III animals received no treatment and represented spontaneous shedders. All eyes were swabbed daily from days 1 through 10 and plated on A549 cells. Recovery of HSV-1 on days 1 through 10 postinduction was analyzed to compare the efficiency of the different methods of viral reactivation.
Reactivation of latent HSV-1 after excimer treatment was observed in nine (45%) of 20 eyes and was equivalent to the rate of reactivation seen in the positive control animals (eight [44.4%] of 18 eyes) (P=.99). Both of these rates were significantly greater than those of the untreated animals (one [5.6%] of 18 eyes) (P=.018).
Excimer laser keratectomy appears to be an efficient trigger for the reactivation of latent HSV-1 in the New Zealand rabbit ocular model.
在新西兰兔眼模型中研究准分子激光角膜切削术作为潜伏性单纯疱疹病毒1型（HSV-1）再激活触发因素的作用。目前文献中关于准分子激光光凝后HSV-1再激活的报道存在矛盾。
给新西兰兔每只眼局部接种HSV-1 McKrae株或W株，在第7天记录培养阳性的树枝状角膜炎。在潜伏状态建立后（21天以上），将动物分为三组：第一组动物每只眼进行准分子激光光凝；第二组动物接受基质内注射无菌水作为阳性对照（一种标准方法）；第三组动物不接受治疗，作为自然排毒者。从第1天到第10天每天对所有眼睛进行擦拭，并接种于A549细胞。分析诱导后第1天到第10天HSV-1的恢复情况，以比较不同病毒再激活方法的效率。
在20只眼中，有9只（45%）在准分子激光治疗后出现潜伏性HSV-1再激活，这与阳性对照动物中观察到的再激活率相当（18只眼中有8只[44.4%]）（P = 0.99）。这两个率均显著高于未治疗动物（18只眼中有1只[5.6%]）（P = 0.018）。
在新西兰兔眼模型中，准分子激光角膜切削术似乎是潜伏性HSV-1再激活的有效触发因素。
