使用羰基化合物的组织荧光方法中组织色氨酸单位的反应性。
Reactivity in tissue tryptophyl units in histofluorescence methods using carbonyl compounds.
作者信息
Partanen S
出版信息
Histochemistry. 1976 Jul 19;47(4):339-43. doi: 10.1007/BF00489200.
PMID:955980
Abstract
The contribution of tissue tryptophyl residues, with both amino and carboxyl groups linked to the peptide bonds, to visible fluorescence was studied following various histochemical methods. Tryptophan residues of chymotrypsinogen and trypsinogen exhibited visible fluorescence after (1) combined formaldehyde-HCl vapour, (2) combined formaldehyde and acetyl chloride vapour, and (3) glyoxylic acid vapour treatment.
摘要
通过各种组织化学方法，研究了组织中色氨酸残基（其氨基和羧基均与肽键相连）对可见荧光的贡献。在以下处理后，胰凝乳蛋白酶原和胰蛋白酶原的色氨酸残基呈现出可见荧光：（1）甲醛 - 盐酸混合蒸汽处理；（2）甲醛与乙酰氯混合蒸汽处理；（3）乙醛酸蒸汽处理。
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