• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用羰基化合物的组织荧光方法中组织色氨酸单位的反应性。

Reactivity in tissue tryptophyl units in histofluorescence methods using carbonyl compounds.

作者信息

Partanen S

出版信息

Histochemistry. 1976 Jul 19;47(4):339-43. doi: 10.1007/BF00489200.

DOI:10.1007/BF00489200
PMID:955980
Abstract

The contribution of tissue tryptophyl residues, with both amino and carboxyl groups linked to the peptide bonds, to visible fluorescence was studied following various histochemical methods. Tryptophan residues of chymotrypsinogen and trypsinogen exhibited visible fluorescence after (1) combined formaldehyde-HCl vapour, (2) combined formaldehyde and acetyl chloride vapour, and (3) glyoxylic acid vapour treatment.

摘要

通过各种组织化学方法，研究了组织中色氨酸残基（其氨基和羧基均与肽键相连）对可见荧光的贡献。在以下处理后，胰凝乳蛋白酶原和胰蛋白酶原的色氨酸残基呈现出可见荧光：（1）甲醛 - 盐酸混合蒸汽处理；（2）甲醛与乙酰氯混合蒸汽处理；（3）乙醛酸蒸汽处理。

相似文献

1
Reactivity in tissue tryptophyl units in histofluorescence methods using carbonyl compounds.使用羰基化合物的组织荧光方法中组织色氨酸单位的反应性。
Histochemistry. 1976 Jul 19;47(4):339-43. doi: 10.1007/BF00489200.
2
Quantitative fluorescence histochemistry of combined formaldehyde-chloral-induced fluorescence of amino-terminal tryptophyl-peptide in model experiments and in the pars intermedia of the rat hypophysis.模型实验及大鼠垂体中间部中联合甲醛-氯醛诱导的氨基末端色氨酰肽荧光的定量荧光组织化学
Histochemistry. 1979 Jul 11;61(3):291-300. doi: 10.1007/BF00508450.
3
Simultaneous fluorescence histochemical demonstration of catecholamines and tryptophyl-peptides in endocrine cells.内分泌细胞中儿茶酚胺和色氨酸肽的同步荧光组织化学显示
Histochemistry. 1975 Jun 9;43(4):295-303. doi: 10.1007/BF00490188.
4
Combined formaldehyde and acetyl chloride vapour condensation: a new fluorescence histochemical method for the demonstration of tryptophyl-peptide-containing endocrine cells of the hypophysis.甲醛与乙酰氯蒸汽联合冷凝法：一种用于显示垂体中含色氨酸肽内分泌细胞的新型荧光组织化学方法。
Histochemistry. 1974;41(2):119-31. doi: 10.1007/BF00499127.
5
Isolation and thin-layer chromatographic identification of several peptides with NH2-terminal tryptophan and their histochemical demonstration in the ACTH cells of the rat hypophysis.几种N端为色氨酸的肽的分离、薄层色谱鉴定及其在大鼠垂体促肾上腺皮质激素细胞中的组织化学显示
Histochemistry. 1978 Jun 9;56(2):147-54. doi: 10.1007/BF00508440.
6
Formaldehyde-hydrochloric acid treatment. A fluorescence histochemical method for the demonstration of tryptophan residues in peptides and proteins.甲醛-盐酸处理。一种用于显示肽和蛋白质中色氨酸残基的荧光组织化学方法。
J Histochem Cytochem. 1975 Dec;23(12):873-81. doi: 10.1177/23.12.1104707.
7
Fluorescence histochemical demonstration of peptides with NH 2 - or COOH-terminal tryptophan or dopa by condensation with glyoxylic acid.通过与乙醛酸缩合对具有NH₂-或COOH-末端色氨酸或多巴的肽进行荧光组织化学演示。
J Histochem Cytochem. 1973 Mar;21(3):253-65. doi: 10.1177/21.3.253.
8
Letter: A new histochemical method for the demonstration of tissue tryptophyl units by 2-acylation and ring closure.信函：一种通过2-酰化和环化来显示组织色氨酸单元的新组织化学方法。
Histochem J. 1973 Nov;5(6):577-8. doi: 10.1007/BF01012063.
9
An improved histofluorescence procedure for freeze-dried paraffin-embedded tissue based on combined formaldehyde-glyoxylic acid perfusion with high magnesium content and acid pH.一种基于高镁含量和酸性pH值的甲醛-乙醛酸联合灌注对冻干石蜡包埋组织的改良组织荧光程序。
Histochemistry. 1976 Oct 29;49(3):177-92. doi: 10.1007/BF00492374.
10
Formaldehyde replacement with glyoxylic acid in semipermanent hair straightening: a new and multidisciplinary investigation.半永久性直发中用乙醛酸替代甲醛：一项新的多学科研究。
Int J Cosmet Sci. 2014 Oct;36(5):459-70. doi: 10.1111/ics.12148. Epub 2014 Aug 11.

引用本文的文献

1
Isolation and thin-layer chromatographic identification of several peptides with NH2-terminal tryptophan and their histochemical demonstration in the ACTH cells of the rat hypophysis.几种N端为色氨酸的肽的分离、薄层色谱鉴定及其在大鼠垂体促肾上腺皮质激素细胞中的组织化学显示
Histochemistry. 1978 Jun 9;56(2):147-54. doi: 10.1007/BF00508440.
2
The endocrine nature of the paranganglia of man.人类副神经节的内分泌性质。
Experientia. 1978 Jan 15;34(1):111-3. doi: 10.1007/BF01921934.
3
Quantitative fluorescence histochemistry of combined formaldehyde-chloral-induced fluorescence of amino-terminal tryptophyl-peptide in model experiments and in the pars intermedia of the rat hypophysis.

本文引用的文献

1
The histochemical demonstration of indole derivatives by the post-coupled p-dimethylaminobenzylidene reaction.通过后偶联对二甲氨基苯亚甲基反应进行吲哚衍生物的组织化学显示。
J Histochem Cytochem. 1957 May;5(3):279-96. doi: 10.1177/5.3.279.
2
Histochemical demonstration of tryptamine. Properties of the formaldehyde-induced fluorophores of tryptamine and related indole compounds in models.色胺的组织化学证明。模型中色胺及相关吲哚化合物甲醛诱导荧光团的性质。
Acta Physiol Scand Suppl. 1968;318:1-31. doi: 10.1111/j.1748-1716.1968.tb04318.x.
3
Histochemical characterization of a tryptamine-like substance stored in cells of the mammalian adenohypophysis.
模型实验及大鼠垂体中间部中联合甲醛-氯醛诱导的氨基末端色氨酰肽荧光的定量荧光组织化学
Histochemistry. 1979 Jul 11;61(3):291-300. doi: 10.1007/BF00508450.
对储存在哺乳动物腺垂体细胞中的一种色胺样物质的组织化学特性分析。
Acta Physiol Scand. 1969 Dec;77(4):475-89. doi: 10.1111/j.1748-1716.1969.tb04591.x.
4
Acid catalysis of the formaldehyde condensation reaction for a sensitive histochemical demonstration of tryptamines and 3-methoxylated phenylethylamines. 2. Characterization of amine fluorophores and application to tissues.用于色胺和3-甲氧基苯乙胺灵敏组织化学显示的甲醛缩合反应的酸催化。2. 胺荧光团的表征及其在组织中的应用。
J Histochem Cytochem. 1971 May;19(5):286-98. doi: 10.1177/19.5.286.
5
The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence.通过甲醛诱导荧光对儿茶酚胺进行实际组织化学显示。
J R Microsc Soc. 1967;87(2):259-76.
6
Letter: A new histochemical method for the demonstration of tissue tryptophyl units by 2-acylation and ring closure.信函：一种通过2-酰化和环化来显示组织色氨酸单元的新组织化学方法。
Histochem J. 1973 Nov;5(6):577-8. doi: 10.1007/BF01012063.
7
Fluorescence histochemical demonstration of peptides with NH 2 - or COOH-terminal tryptophan or dopa by condensation with glyoxylic acid.通过与乙醛酸缩合对具有NH₂-或COOH-末端色氨酸或多巴的肽进行荧光组织化学演示。
J Histochem Cytochem. 1973 Mar;21(3):253-65. doi: 10.1177/21.3.253.
8
Glyoxylic acid condensation: a new fluorescence method for the histochemical demonstration of biogenic monoamines.乙醛酸缩合反应：一种用于生物源性单胺组织化学显示的新荧光方法。
Acta Physiol Scand. 1973 Jan;87(1):57-62. doi: 10.1111/j.1748-1716.1973.tb05365.x.
9
Tryptamine or tryptophyl peptides in endocrine cells of the mammalian adenohypophysis?哺乳动物腺垂体内分泌细胞中的色胺或色氨酸肽？
J Histochem Cytochem. 1972 Nov;20(11):908-16. doi: 10.1177/20.11.908.
10
Combined formaldehyde and acetyl chloride vapour condensation: a new fluorescence histochemical method for the demonstration of tryptophyl-peptide-containing endocrine cells of the hypophysis.甲醛与乙酰氯蒸汽联合冷凝法：一种用于显示垂体中含色氨酸肽内分泌细胞的新型荧光组织化学方法。
Histochemistry. 1974;41(2):119-31. doi: 10.1007/BF00499127.