Kaufmann R, Patt S, Schafberg H, Kalff R, Neupert G, Nowak G
Research Group Pharmacological Hemostaseology, Medical Faculty, Friedrich Schiller University Jena, Germany.
Neuroreport. 1998 Mar 9;9(4):709-12. doi: 10.1097/00001756-199803090-00027.
In this study we investigated primary cultures obtained from two glioblastomas surgically removed from a 64-year-old man and a 50-year-old woman, respectively. The presence of the tethered ligand thrombin receptor PAR1 (protease-activated receptor 1) in these cells was demonstrated at the level of receptor binding by using immunofluorescence studies with the monoclonal anti-PAR1 antibody Mab 31-2. Stimulation of human glioblastoma cells both with alpha-thrombin and the thrombin receptor activating peptide TRAP-6 resulted in a series of [Ca+]i spikes as shown by confocal laser fluorescence microscopy with fluo-3 as calcium sensitive fluorescence indicator. This effect was completely blocked with the thrombin receptor antagonist peptide T1. Our results demonstrate functional thrombin receptors (PAR1) in primary cultures of human glioblastomas for the first time.
在本研究中，我们分别对从一名64岁男性和一名50岁女性身上手术切除的两个胶质母细胞瘤获得的原代培养物进行了研究。通过使用单克隆抗PAR1抗体Mab 31-2进行免疫荧光研究，在受体结合水平证实了这些细胞中存在锚定配体凝血酶受体PAR1（蛋白酶激活受体1）。用α-凝血酶和凝血酶受体激活肽TRAP-6刺激人胶质母细胞瘤细胞，如用fluo-3作为钙敏感荧光指示剂的共聚焦激光荧光显微镜所示，导致一系列[Ca+]i峰值。凝血酶受体拮抗剂肽T1完全阻断了这种效应。我们的结果首次证明了人胶质母细胞瘤原代培养物中存在功能性凝血酶受体（PAR1）。
