Naito T, Saito Y, Yamamoto J, Nozaki Y, Tomura K, Hazama M, Nakanishi S, Brenner S
Pharmaceutical Frontier Research Laboratories, Japan Tobacco, Inc., 13-2 Fukuura 1-chome, Kanazawa-ku, Yokohama, Kanagawa 236 Japan.
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5178-81. doi: 10.1073/pnas.95.9.5178.
By a combination of PCR with degenerate primers and low stringency probing, we have isolated a large family of genes related to the Ca2+-sensing receptor from the genome of Fugu rubripes. One of the genes (type I) is the Fugu homologue of the Ca2+-sensing receptor. The remaining genes can be divided into five classes (type II-VI) on the bases of gene structure. In several types, the genes occur in clusters as tandem arrays. These genes appear to be the homologues of the vomeronasal pheromone receptors recently described in rodents. The Fugu genes are expressed in the tissues of the nose, suggesting that they may have a similar physiological role.
通过使用简并引物进行聚合酶链反应（PCR）和低严谨度探针杂交相结合的方法，我们从红鳍东方鲀的基因组中分离出了一大类与钙离子感受受体相关的基因。其中一个基因（I型）是钙离子感受受体的红鳍东方鲀同源物。其余的基因根据基因结构可分为五类（II - VI型）。在几种类型中，这些基因以串联阵列的形式成簇出现。这些基因似乎是最近在啮齿动物中描述的犁鼻器信息素受体的同源物。红鳍东方鲀的这些基因在鼻子组织中表达，这表明它们可能具有相似的生理作用。
