Murphy J A, Thompson M R, Pappelis A J
J Bacteriol. 1976 Sep;127(3):1465-71. doi: 10.1128/jb.127.3.1465-1471.1976.
The ultrastructure of Diplodia maydis spores was studied in thin sections with a transmission electron microscope. Storage vacuoles were evenly distributed in the two cells. Some of the vacuoles that contained a dense osmiophilic sphere(s) were surrounded by a membrane, and had membranous aggregates around their periphery. The sport wall was composed of an electron-dense layer and an electron-translucent layer. An inner cytoplasmic membrane was present. Dormant and germinating spores were studied with scanning electron microscopy and also with a Si (Li) energy-dispersive X-ray analyzer. The dormant spore was ovate and usually two-celled with a central septum. Germination proceeded via a germ tube from the side of one end of the cell. Of several methods for preparation of specimens for X-ray analysis studied, freeze-dried spores mounted on carbon stubs and then further carbon coated gave the best results. X-ray analyses revealed that spore populations contained large amounts of Si, P, Cl, and K, smaller amounts of S and Ca, and trace amounts of Mg and Al. Analyses of single spores revealed high K and Cl and low P and Mg at one end of the cell with concomitant low K and Cl and high P and Mg in the central portion and other end of the cell. In two-celled germinating spores, high K and Cl occurred in the end of the nongerminating spore cell, whereas the germinating cell contained high P and Mg and low K and Cl. X-ray image maps revealed that K and Cl were located together at one end of the spore.
利用透射电子显微镜对玉米 Diplodia maydis 孢子的超微结构进行了研究。贮藏液泡均匀分布在两个细胞中。一些含有致密嗜锇球体的液泡被膜包围，其周围有膜状聚集体。孢子壁由电子致密层和电子半透明层组成。存在一层内膜。利用扫描电子显微镜以及硅（锂）能量色散 X 射线分析仪对休眠孢子和萌发孢子进行了研究。休眠孢子呈卵形，通常为两细胞，有中央隔膜。萌发通过细胞一端侧面的芽管进行。在所研究的几种用于 X 射线分析的标本制备方法中，将冻干孢子安装在碳柱上，然后进一步进行碳涂层处理，效果最佳。X 射线分析表明，孢子群体含有大量的硅、磷、氯和钾，少量的硫和钙，以及微量的镁和铝。对单个孢子的分析显示，细胞一端钾和氯含量高，磷和镁含量低，而细胞中部和另一端钾和氯含量低，磷和镁含量高。在两细胞萌发孢子中，未萌发孢子细胞的一端钾和氯含量高，而萌发细胞中磷和镁含量高，钾和氯含量低。X 射线图像图谱显示，钾和氯位于孢子的一端。
