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不同分类群四膜虫中的线粒体脱氢酶:胰岛素的作用

Mitochondrial dehydrogenases in different taxa of tetrahymena: effect of insulin.

作者信息

Kóhidai L, Csaba G

机构信息

Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.

出版信息

Biosci Rep. 1997 Dec;17(6):529-35. doi: 10.1023/a:1027308223168.

DOI:10.1023/a:1027308223168
PMID:9561297
Abstract

Mitochondrial dehydrogenase activity was measured in seven taxa of Tetrahymena (T. pyriformis G1. T. hegewishi, T. malaccensis, T. pigmentosa, T. shapiro, T. thermophila CU-399, T. thermophila MS-1). Enzyme activity was different in the taxa investigated. Insulin reduced enzyme activity in six of the seven taxa studied. The duration of activity reduction was relatively long (5-10 min.) in most of the cases, and in T. hegewishi this lasted up to the end of the measurements (30 min.). There was no interrelation between the basic dehydrogenase activity of the taxon and the effect of insulin. There was also no correlation between the degree of relationship (of the taxa) and the dehydrogenase profile after insulin treatment.

摘要

在七种四膜虫分类群(梨形四膜虫G1、赫格维希四膜虫、马六甲四膜虫、色素四膜虫、夏皮罗四膜虫、嗜热四膜虫CU - 399、嗜热四膜虫MS - 1)中测定了线粒体脱氢酶活性。在所研究的分类群中,酶活性存在差异。胰岛素降低了所研究的七个分类群中六个分类群的酶活性。在大多数情况下,活性降低的持续时间相对较长(5 - 10分钟),而在赫格维希四膜虫中,这种情况一直持续到测量结束(30分钟)。分类群的基本脱氢酶活性与胰岛素的作用之间没有相互关系。(分类群之间的)亲缘关系程度与胰岛素处理后的脱氢酶谱之间也没有相关性。

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