神经生长因子-β、神经内分泌细胞与前列腺癌细胞系。神经生长因子-β刺激后人前列腺癌细胞系DU-145、PC-3、LNCaP和TSU-pr1中神经内分泌表达的研究。

NGF-beta, NE-cells and prostatic cancer cell lines. A study of neuroendocrine expression in the human prostatic cancer cell lines DU-145, PC-3, LNCaP, and TSU-pr1 following stimulation of the nerve growth factor-beta.

作者信息

Angelsen A, Sandvik A K, Syversen U, Stridsberg M, Waldum H L

机构信息

Department of Physiology and Biomedical Engineering, Norwegian University of Technology and Science, Norway.

出版信息

Scand J Urol Nephrol. 1998 Feb;32(1):7-13. doi: 10.1080/003655998750014611.

DOI:10.1080/003655998750014611

PMID:9561567

Abstract

Neuroendocrine (NE) cells are present in both benign and malignant human prostate. However, their function is poorly understood, mainly due to the lack of suitable experimental models. The nerve growth factor-beta (NGF-beta) promotes the rat pheochromocytoma cell line PC-12 to differentiate into neuronal like cells. We have studied the effect of NGF-beta on four human prostate cancer cell lines, LNCaP, DU-145, PC-3, and TSU-pr1. NGF-beta stimulates the growth rate in all these cell lines, but does not induce a neuronal phenotype. NE tumour markers (chromogranin A [CgA] and chromogranin B[CgB]) could not be demonstrated by immunocytochemistry (CgA and CgB), Northern blotting (CgA), or ELISA techniques (CgA), neither in control nor in NGF-beta stimulated cells. Consequently, other experimental models have to be sought in the study of NE cells in the human prostate.

摘要

神经内分泌（NE）细胞存在于人类良性和恶性前列腺组织中。然而，其功能却鲜为人知，主要原因是缺乏合适的实验模型。神经生长因子-β（NGF-β）可促使大鼠嗜铬细胞瘤细胞系PC-12分化为神经元样细胞。我们研究了NGF-β对四种人前列腺癌细胞系LNCaP、DU-145、PC-3和TSU-pr1的影响。NGF-β可刺激所有这些细胞系的生长速率，但不会诱导神经元表型。无论是在对照细胞还是在NGF-β刺激的细胞中，通过免疫细胞化学（嗜铬粒蛋白A [CgA]和嗜铬粒蛋白B [CgB]）、Northern印迹法（CgA）或酶联免疫吸附测定技术（CgA）均无法检测到NE肿瘤标志物。因此，在研究人前列腺中的NE细胞时，必须寻找其他实验模型。