Cicalese L, Nüssler N C, Hoffman R A, Neuhaus P, Schraut W H
Department of Surgery, University of Pittsburgh, PA 15261, USA.
Transpl Int. 1998;11(2):102-9. doi: 10.1007/s001470050112.
Infiltration of a transplanted organ by host lymphoid cells is the hallmark of acute rejection. However, after intestinal transplantation, physiological lymphocyte migration may lead to host cell infiltration of the graft even in the absence of rejection. It is unclear whether this lymphocyte migration also involves the intraepithelial compartment of the graft or whether infiltration there is indicative of acute rejection. We demonstrate here that host cell infiltration of the intestinal mucosa occurs both during acute rejection of a small bowel allograft and, to a lesser extent, when rejection is prevented by immunosuppression with FK506. The infiltrating host cells consisted of CD3+ T cells with a predominant CD4-CD8+ phenotype resembling intraepithelial lymphocytes (IELs). Functional studies showed that the nonspecific cytolytic activity of IELs was not affected by acute rejection or by immunosuppression with FK506. These findings indicate that host cell infiltration of the intestinal mucosa does not connote an ongoing acute rejection. Furthermore, the decreased mucosal barrier function during acute rejection of intestinal allgrafts is probably not due to impaired cytolytic activity of IELs.
宿主淋巴细胞浸润移植器官是急性排斥反应的标志。然而，肠道移植后，即使在没有排斥反应的情况下，生理性淋巴细胞迁移也可能导致宿主细胞浸润移植物。目前尚不清楚这种淋巴细胞迁移是否也涉及移植物的上皮内部分，或者那里的浸润是否表明急性排斥反应。我们在此证明，小肠同种异体移植急性排斥反应期间以及在较小程度上，当用FK506免疫抑制预防排斥反应时，都会发生宿主细胞浸润肠黏膜。浸润的宿主细胞由CD3 + T细胞组成，其主要CD4 - CD8 +表型类似于上皮内淋巴细胞（IEL）。功能研究表明，IEL的非特异性细胞溶解活性不受急性排斥反应或FK506免疫抑制的影响。这些发现表明，宿主细胞浸润肠黏膜并不意味着正在发生急性排斥反应。此外，肠道同种异体移植急性排斥反应期间黏膜屏障功能下降可能并非由于IEL的细胞溶解活性受损。
