白色念珠菌苯丙氨酰-tRNA合成酶β亚基基因的克隆与鉴定

Cloning and characterization of the phenylalanyl-tRNA synthetase beta subunit gene from Candida albicans.

作者信息

Marcilla A, Pallotti C, Gomez-Lobo M, Caballero P, Valentin E, Sentandreu R

机构信息

Secció Departamental de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Burjassot, Valencia, Spain.

出版信息

FEMS Microbiol Lett. 1998 Apr 1;161(1):179-85. doi: 10.1111/j.1574-6968.1998.tb12946.x.

DOI:10.1111/j.1574-6968.1998.tb12946.x

PMID:9561746

Abstract

A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.

摘要

从再生原生质体分离的RNA构建了白色念珠菌表达文库。使用一个1.4kb的cDNA克隆分离出一个基因组片段。序列分析显示一个由593个氨基酸组成的开放阅读框，与酿酒酵母的苯丙氨酰-tRNA合成酶β亚基（FRS1）总体一致性为63.6%。我们将其命名为CaFRS1。它以单拷贝形式位于染色体R的SfiI片段M中。其表达在白色念珠菌酵母细胞和菌丝体细胞的原生质体细胞壁再生过程中均呈现下降，表明其在细胞壁合成的初始步骤中是必需的。