伤寒沙门氏菌rpoS突变体的细胞毒性低于亲本菌株，但能在静息的THP-1巨噬细胞内存活。
Salmonella typhi rpoS mutant is less cytotoxic than the parent strain but survives inside resting THP-1 macrophages.
作者信息
Khan A Q, Zhao L, Hirose K, Miyake M, Li T, Hashimoto Y, Kawamura Y, Ezaki T
机构信息
Department of Microbiology, Gifu University School of Medicine, Japan.
出版信息
FEMS Microbiol Lett. 1998 Apr 1;161(1):201-8. doi: 10.1111/j.1574-6968.1998.tb12949.x.
Transcription of the stationary-phase sigma factor RpoS of Salmonella typhi increased in the macrophage. A single rpoS mutant of S. typhi was constructed to analyze the role of RpoS in intracellular multiplication of the bacterium and host cell killing. This mutant was sensitive to starvation, low pH and hydrogen peroxide; however, it could still multiply inside resting macrophages and was less cytotoxic than the wild-type strain. Therefore, S. typhi might produce RpoS-dependent factors which could contribute to host cell death.
伤寒沙门氏菌的稳定期σ因子RpoS在巨噬细胞中的转录增加。构建了一株伤寒沙门氏菌的rpoS单突变体，以分析RpoS在该细菌细胞内增殖及杀伤宿主细胞中的作用。该突变体对饥饿、低pH值和过氧化氢敏感；然而，它仍能在静息巨噬细胞内增殖，且细胞毒性比野生型菌株小。因此，伤寒沙门氏菌可能产生依赖RpoS的因子，这些因子可能导致宿主细胞死亡。
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