Lavens-Phillips S E, Mockford E H, Warner J A
Johns Hopkins Asthma & Allergy Center, Division of Clinical Immunology, Baltimore, MD 21224, USA.
Inflamm Res. 1998 Mar;47(3):137-43. doi: 10.1007/s000110050303.
To investigate the role of tyrosine kinases (TK) in IgE-mediated signal transduction in human lung mast cells (HLMC) and basophils.
Peripheral blood basophils (n > or = 4) and human lung mast cells (n > or = 6).
Cells were preincubated with TK inhibitor for 15 min at 37 degrees C, before the addition of anti-IgE.
Histamine release (HR) was assayed using a fluorimetric technique. Results were compared using nonparametric statistics.
Piceatannol and ST638 significantly (p < or = 0.05) inhibited anti-IgE induced HR from HLMCs and basophils whilst lavendustin C had no effect in either cell type. Herbimycin A also significantly (p < or = 0.05) inhibited anti-IgE induced HR from both cell types, an effect which was dose dependent but did require a 16 h preincubation with drug.
In summary, HLMCs and basophils exhibit distinct inhibitory profiles in the presence of various inhibitors of TK.
