Hu Y, Yang M, Ma N, Shinohara H, Semba R
Department of Anatomy, Mie University School of Medicine, Tsu, Japan.
Histochem Cell Biol. 1998 Apr;109(4):369-73. doi: 10.1007/s004180050237.
Recent studies have shown that carbon monoxide (CO) may function as a gaseous signaling molecule in a similar way to nitric oxide. In the gastrointestinal tract, immunoreactivity against a CO-producing enzyme, heme oxygenase-2 (HO-2), was reported in epithelial cells and neurons of submucosal and myenteric plexus. However, details of the epithelial cells in the gastric mucosa remain unknown. The aim of this study was to clarify if mRNA for HO-2 is expressed in the rat stomach, if HO-2 protein is present in the mucosa, and to define the cell types of the HO-2-immunoreactive cells. HO-2 mRNA and protein were detected in fundic and pyloric mucosa of rat stomach using an RNA protection assay and western blot analysis. Immunohistochemical study showed that HO-2 was localized in parietal cells of the fundic glands and gastrin cells of the pyloric glands of both rat and monkey. The results suggest that HO-2 enzyme is produced in the gastric mucosa, and that CO is released from parietal cells and gastrin cells.
最近的研究表明，一氧化碳（CO）可能作为一种气体信号分子，其作用方式与一氧化氮类似。在胃肠道中，据报道，在黏膜下神经丛和肌间神经丛的上皮细胞和神经元中存在针对一种产生CO的酶——血红素加氧酶-2（HO-2）的免疫反应性。然而，胃黏膜上皮细胞的具体情况仍不清楚。本研究的目的是阐明HO-2的mRNA在大鼠胃中是否表达，黏膜中是否存在HO-2蛋白，并确定HO-2免疫反应性细胞的细胞类型。使用RNA保护分析和蛋白质印迹分析在大鼠胃的胃底和幽门黏膜中检测到了HO-2的mRNA和蛋白。免疫组织化学研究表明，HO-2定位于大鼠和猴的胃底腺壁细胞和幽门腺胃泌素细胞中。结果表明，胃黏膜中产生HO-2酶，并且CO从壁细胞和胃泌素细胞中释放。
