Zakarija M, McKenzie J M
J Clin Endocrinol Metab. 1978 Oct;47(4):906-8. doi: 10.1210/jcem-47-4-906.
Immunoglobulin G purified from the serum of patients with Graves' disease, stimulated the thyroid of man, calf, and guinea pig (cAMP accumulation as the end-point) in vitro, and the thyroid of the mouse in vivo (LATS bioassay). The stimulatory effect on the thyroids of all four species was removed by adsorption of the immunoglobulin G to human thyroid membranes and was diminished to a proportionately similar degree by adsorption with bovine thyroid membranes. All activity, as assessed by stimulation of the human thyroid in vitro. was recovered from both human and bovine membranes by elution with 2 M NaSCN solution. The data support the concept that the thyroid-stimulating antibody of Graves' disease is homologous to a human thyroid antigen and, in some instances, cross-reacts with a similar antigen in the thyroid of a distant species and so stimulates the heterologous gland.
从格雷夫斯病患者血清中纯化的免疫球蛋白G，在体外能刺激人、小牛和豚鼠的甲状腺（以环磷酸腺苷积累为终点），在体内能刺激小鼠的甲状腺（长效甲状腺刺激素生物测定法）。通过将免疫球蛋白G吸附到人甲状腺膜上，对所有这四种物种甲状腺的刺激作用均被消除，而用牛甲状腺膜吸附后，刺激作用则以成比例的相似程度减弱。通过体外刺激人甲状腺来评估，所有活性均通过用2M硫氰酸钠溶液洗脱，从人膜和牛膜中回收。这些数据支持这样一种概念，即格雷夫斯病的促甲状腺抗体与人甲状腺抗原同源，并且在某些情况下，与远缘物种甲状腺中的类似抗原发生交叉反应，从而刺激异种腺体。
