Hall M, Mallen D N
J Chromatogr Sci. 1976 Sep;14(9):451-2. doi: 10.1093/chromsci/14.9.451.
Benefin (N-[n-butyl]-N-ethyl-2,6-dinitro-alpha, alpha, alpha-trifluoro-p-toluidine) and trifluralin (2,6-dinitro-N,N-di-n-propyl-alpha, alpha, alpha-trifluoro-p-toluidine) (Figure 1) are isomeric preemergent herbicides (1,2). As they possess different activities, their use necessitates the development of a routine analytical method which differentiates between them. The separation of these isomers by gas-liquid chromatography (GLC) using conventional column packings has proved extremely difficult. Two partly successful separations have been previously reported to date. A partial separation was obtained using a Durapak-Carbowax 400/Poracil C column (3) and a complete separation using a column packed with a monomolecular layer of Carbowax 20M on Chromosorb W (4). Unfortunately, this latter column has a very limited life and the analysis time is 25 minutes. This paper reports a rapid and reproducible separation of benefin and trifluralin using a liquid crystal stationary phase.
乙丁氟灵（N-（正丁基）-N-乙基-2,6-二硝基-α,α,α-三氟对甲苯胺）和氟乐灵（2,6-二硝基-N,N-二正丙基-α,α,α-三氟对甲苯胺）（图1）是同分异构的芽前除草剂（1,2）。由于它们具有不同的活性，因此在使用时需要开发一种能够区分它们的常规分析方法。使用传统柱填料通过气液色谱法（GLC）分离这些异构体已证明极其困难。迄今为止，此前已有两篇部分成功分离的报道。使用Durapak-聚乙二醇400/硅胶C柱实现了部分分离（3），使用在Chromosorb W上填充有单分子层聚乙二醇20M的柱实现了完全分离（4）。不幸的是，后一种柱的使用寿命非常有限，且分析时间为25分钟。本文报道了使用液晶固定相对乙丁氟灵和氟乐灵进行快速且可重复的分离。
