Le P U, Nguyen T N, Drolet-Savoie P, Leclerc N, Nabi I R
Département de Pathologie et Biologie Cellulaire, Université de Montréal, Québec, Canada.
Cancer Res. 1998 Apr 15;58(8):1631-5.
An invasive variant of Moloney sarcoma virus-transformed MDCK cells (MSV-MDCK-INV), which was isolated by the repeated selection of cells that successfully traversed a Matrigel-coated filter, exhibits increased motile ability and presents an elongated cell shape and numerous pseudopodia. Although stress fibers are present in both MDCK and MSV-MDCK cells, MSV-MDCK-INV cells contain no stress fibers and exhibit a dense concentration of actin at the tips of pseudopodia. Relative to both MDCK and MSV-MDCK cells, the MSV-MDCK-INV cells exhibit increased expression of beta-actin and redistribution of beta-actin to the tips of pseudopodia. These actin concentrations are enriched in both F- and G-actin and, thus, represent dynamic regions of actin cytoskeleton remodeling. The acquisition of invasive properties by epithelial transformants is, therefore, associated with the increased expression of beta-actin and its concentration in actin-rich domains, which may drive pseudopodial extension and facilitate tumor cell invasion.
莫洛尼肉瘤病毒转化的MDCK细胞（MSV-MDCK-INV）的侵袭性变体是通过反复筛选成功穿过基质胶包被滤膜的细胞而分离得到的，它表现出增强的运动能力，细胞形态拉长且有许多伪足。尽管MDCK细胞和MSV-MDCK细胞中都存在应力纤维，但MSV-MDCK-INV细胞中没有应力纤维，且在伪足尖端呈现肌动蛋白的密集聚集。相对于MDCK细胞和MSV-MDCK细胞，MSV-MDCK-INV细胞中β-肌动蛋白的表达增加，且β-肌动蛋白重新分布到伪足尖端。这些肌动蛋白聚集区富含F-肌动蛋白和G-肌动蛋白，因此代表了肌动蛋白细胞骨架重塑的动态区域。因此，上皮转化细胞获得侵袭特性与β-肌动蛋白表达增加及其在富含肌动蛋白结构域中的聚集有关，这可能驱动伪足延伸并促进肿瘤细胞侵袭。
