Hammond E M, Brunet C L, Johnson G D, Parkhill J, Milner A E, Brady G, Gregory C D, Grand R J
CRC Institute for Cancer Studies, The University of Birmingham, Edgbaston, UK.
FEBS Lett. 1998 Apr 3;425(3):391-5. doi: 10.1016/s0014-5793(98)00266-x.
Apoptosis specific proteins (ASP) are expressed in the cytoplasm of cultured mammalian cells of various lineages following induction of apoptosis. The cDNA encoding ASP has been cloned from a human expression library and has significant homology to the Saccharomyces cerevisiae APG5 gene which is essential for yeast autophagy. The ASP gene, known as hAPG5, can be transcribed to give mRNAs of 3.3 kbp, 2.5 kbp and 1.8 kbp which are present at comparable levels in viable and apoptotic cells, demonstrating that protein expression must be regulated at the translational level. These data indicate a possible relationship between apoptosis and autophagy and suggest evolutionary conservation in mammalian apoptosis of a degradative process present in yeast.
凋亡特异性蛋白（ASP）在诱导凋亡后，在各种谱系的培养哺乳动物细胞的细胞质中表达。编码ASP的cDNA已从人表达文库中克隆出来，与酿酒酵母APG5基因具有显著同源性，该基因对酵母自噬至关重要。ASP基因，即hAPG5基因，可转录产生3.3kbp、2.5kbp和1.8kbp的mRNA，这些mRNA在活细胞和凋亡细胞中的水平相当，表明蛋白质表达必须在翻译水平上受到调控。这些数据表明凋亡与自噬之间可能存在关系，并提示酵母中存在的一种降解过程在哺乳动物凋亡中具有进化保守性。
