Randez-Gil F, Herrero P, Sanz P, Prieto J A, Moreno F
Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Burjassot Valencia, Spain.
FEBS Lett. 1998 Apr 3;425(3):475-8. doi: 10.1016/s0014-5793(98)00289-0.
We describe here that the HXK2 gene product, isoenzyme PII of hexokinase, is located in both the nucleus and the cytoplasm of Saccharomyces cerevisiae cells. This conclusion is supported by assays of hexokinase-specific activity in isolated nuclei from wild-type and hxk1lhxk2 double mutant strains, by immunoblot experiments using anti-Hxk2 antibodies and by observation of the fluorescence distribution of a Hxk2-GFP fusion protein in cells transformed with the HXK2::gfp gene.
我们在此描述，己糖激酶的同工酶PII即HXK2基因产物，位于酿酒酵母细胞的细胞核和细胞质中。来自野生型和hxk1/hxk2双突变菌株的分离细胞核中的己糖激酶特异性活性测定、使用抗Hxk2抗体的免疫印迹实验以及对用HXK2::gfp基因转化的细胞中Hxk2-GFP融合蛋白荧光分布的观察，均支持这一结论。
