Shimada T, Matsumoto M, Tatsumi Y, Kanamaru A, Akira S
Department of Biochemistry, Hyogo College of Medicine, Nishinomiya, Japan.
FEBS Lett. 1998 Apr 3;425(3):490-4. doi: 10.1016/s0014-5793(98)00299-3.
To identify genes induced in activated macrophages, we screened a cDNA library prepared from the lipopolysaccharide (LPS)-treated cell line, RAW264, using the suppression subtractive hybridization technique. One of the clones isolated was dramatically induced by LPS in macrophages. The predicted protein sequence of this gene contains the domain unique to seven transmembrane receptors, and shows similarity with mouse C-C chemokine receptor 5 (CCR5). Therefore, we designated it LPS inducible C-C chemokine receptor related gene (L-CCR). Northern blot analysis revealed that L-CCR was specifically expressed in differentiated macrophages after LPS stimulation. These results show that L-CCR is a novel C-C chemokine receptor related gene induced by LPS in macrophages and may play an important role in inflammatory responses.
为了鉴定在活化巨噬细胞中诱导表达的基因，我们使用抑制性消减杂交技术，筛选了一个由脂多糖（LPS）处理的细胞系RAW264制备的cDNA文库。分离得到的一个克隆在巨噬细胞中被LPS显著诱导。该基因预测的蛋白质序列包含七跨膜受体特有的结构域，并且与小鼠C-C趋化因子受体5（CCR5）具有相似性。因此，我们将其命名为LPS诱导的C-C趋化因子受体相关基因（L-CCR）。Northern印迹分析显示，L-CCR在LPS刺激后的分化巨噬细胞中特异性表达。这些结果表明，L-CCR是LPS在巨噬细胞中诱导产生的一个新的C-C趋化因子受体相关基因，可能在炎症反应中发挥重要作用。
