Hirsch M S, Svoboda K H
Department of Anatomy and Neurobiology, Boston University School of Medicine, MA 02118, USA.
Biotechniques. 1998 Apr;24(4):632-6. doi: 10.2144/98244st04.
A serum-free organ culture model for chondrocyte maturation, using the Avian sternum, was developed. Day-14 chick embryo sterna were placed in organ culture in the presence of defined medium. The optimal medium for chondrocyte terminal differentiation contained specific concentrations of dexamethasone, insulin, thyroid hormone and ascorbic acid. Three parameters, including sternal growth, cell diameter and type X collagen production, were analyzed as indicators of chondrocyte terminal differentiation. These parameters were analyzed in cephalic, middle and caudal regions of the organ-cultured chick sterna and compared to sterna grown in ovo. This study demonstrates that the organ-cultured tissue maintains normal morphological characteristics and terminal differentiation in the cephalic region only, similar to in ovo development, while maintaining normal cell-matrix relationships.
开发了一种使用鸡胸骨的无血清器官培养模型，用于软骨细胞成熟。将第14天鸡胚的胸骨置于含有特定培养基的器官培养中。软骨细胞终末分化的最佳培养基含有特定浓度的地塞米松、胰岛素、甲状腺激素和抗坏血酸。分析了胸骨生长、细胞直径和X型胶原蛋白产生这三个参数，作为软骨细胞终末分化的指标。在器官培养的鸡胸骨的头部、中部和尾部区域分析这些参数，并与在体内生长的胸骨进行比较。这项研究表明，器官培养的组织仅在头部区域保持正常的形态特征和终末分化，类似于体内发育，同时保持正常的细胞-基质关系。
