Tóth T, Papp C, Tóth-Pál E, Nagy B, Papp Z
Department of Obstetrics and Gynaecology, Semmelweis University Medical School, Budapest, Hungary.
J Reprod Med. 1998 Mar;43(3):219-22.
Prenatal determination of fetal rhesus D (RhD) status is desirable in pregnancies in sensitized, RhD-negative women to prevent hydropic degeneration of the fetus. Recently, a polymerase chain reaction (PCR) test on amniocytes or chorionic villi has been in use to demonstrate the RhD status of the fetus in sensitized pregnancies. A more advisable, noninvasive approach is to determine the fetal RhD group from fetal cells circulating in maternal blood.
We report on a prenatal diagnosis where RhD-positive cells could be detected from peripheral blood of a sensitized, RhD-negative mother. The presence of an RhD-positive fetus was confirmed by subsequent amplification of fetal DNA obtained by chorionic villus biopsy.
In sensitized pregnancies, the number of fetal cells in maternal blood seems to be high enough to be detected by PCR in every case.
对于致敏的RhD阴性孕妇，产前确定胎儿的恒河猴D（RhD）状态有助于预防胎儿水肿变性。最近，已采用对羊水细胞或绒毛膜绒毛进行聚合酶链反应（PCR）检测，以确定致敏妊娠中胎儿的RhD状态。一种更可取的非侵入性方法是从母体血液中循环的胎儿细胞来确定胎儿的RhD血型。
我们报告了一例产前诊断，在一名致敏的RhD阴性母亲的外周血中检测到了RhD阳性细胞。通过对绒毛膜绒毛活检获得的胎儿DNA进行后续扩增，证实了RhD阳性胎儿的存在。
在致敏妊娠中，母血中胎儿细胞的数量似乎足够多，以至于在每种情况下都能通过PCR检测到。
