Höglund J, Nilsson O, Ljungström B L, Hellander J, Lind E O, Uggla A
Department of Parasitology, National Veterinary Institute, Uppsala, Sweden.
Vet Parasitol. 1998 Feb 15;75(1):71-9. doi: 10.1016/s0304-4017(97)00188-x.
The egg output and humoral antibody response to scolex antigens of the equine tapeworm Anoplocephala perfoliata were monitored in naturally infected foals by an egg flotation/centrifugation method and an indirect enzyme-linked immunosorbent assay (ELISA). The study was performed on a stud farm in south-western Sweden between May 1994 and April 1995. Sequential blood and faecal samples were taken from 21 foals during their first summer on pasture and until tapeworm eggs were detected. Results were expressed separately for 10 and 11 foals born before and after the end of April 1994, respectively. Increased levels of antibodies were noticed from October and onwards in both groups whereas tapeworm eggs were detected in the faeces of all foals about 4 months later. The antibody response was similar in both groups but it was more pronounced in foals born before April 1994. All foals were treated in March 1995 with an oral paste formulation of pyrantel pamoate at a dose rate of 38 mg/kg bodyweight. Most animals responded to the anthelmintic treatment and one month later, tapeworm eggs were only detected in one out of the 18 foals examined one month after treatment. Thus, the treatment reduced the number of A. perfoliata egg positive horses by 94%. A concomitant decline in antibody levels was also observed. Western immunoblot analysis of sequential individual serum samples showed that at least 10 different scolex antigens in the molecular weight range 10-200 kDa were recognised. Banding intensities, especially of the 10 kDa, 45 kDa and 66 kDa proteins were different in the sequential sera taken during the course of infection.
通过虫卵漂浮/离心法和间接酶联免疫吸附测定（ELISA），对自然感染的幼驹体内叶状裸头绦虫（Anoplocephala perfoliata）头节抗原的虫卵排出量和体液抗体反应进行了监测。该研究于1994年5月至1995年4月在瑞典西南部的一个种马场进行。在21匹幼驹第一年夏季放牧期间直至检测到绦虫卵，连续采集血液和粪便样本。分别对1994年4月底之前和之后出生的10匹和11匹幼驹的结果进行了分析。两组幼驹从10月起抗体水平均升高，而约4个月后在所有幼驹粪便中均检测到绦虫卵。两组的抗体反应相似，但在1994年4月之前出生的幼驹中更为明显。所有幼驹于1995年3月接受了口服吡喹酮糊剂治疗，剂量为38 mg/kg体重。大多数动物对驱虫治疗有反应，治疗1个月后，在接受检查的18匹幼驹中只有1匹在治疗1个月后检测到绦虫卵。因此，治疗使叶状裸头绦虫虫卵阳性马匹数量减少了94%。同时也观察到抗体水平下降。对连续的个体血清样本进行的蛋白质免疫印迹分析表明，至少可识别分子量在10 - 200 kDa范围内的10种不同头节抗原。在感染过程中采集的连续血清中，条带强度，尤其是10 kDa、45 kDa和66 kDa蛋白质的条带强度有所不同。
