Weimer L K, Gamache D A, Yanni J M
Ophthalmology Research, Alcon Laboratories, Fort Worth, Tex 76134, USA.
Int Arch Allergy Immunol. 1998 Apr;115(4):288-93. doi: 10.1159/000069459.
The present studies demonstrate that histamine induces the secretion of IL-6, IL-8 and GM-CSF from human conjunctival epithelial cells in a dose- and time-dependent manner. The histamine antagonists emedastine (H1), ranitidine (H2) and thioperamide (H3) were evaluated for their ability to inhibit secretion of these cytokines. Emedastine potently inhibited histamine-induced IL-6, IL-8 and GM-CSF secretion with mean IC50 values of 2.23, 3.42 and 1.50 nM, respectively. Ranitidine and thioperamide failed to inhibit cytokine secretion over a wide dose range. These data suggest that mast cell derived histamine may stimulate inflammatory cytokine production in allergic conjunctivitis via activation of epithelial cell H1 receptors. The histamine H1 antagonist emedastine potently inhibits this response.
目前的研究表明，组胺以剂量和时间依赖性方式诱导人结膜上皮细胞分泌白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）。评估了组胺拮抗剂依美斯汀（H1）、雷尼替丁（H2）和硫代哌酰胺（H3）抑制这些细胞因子分泌的能力。依美斯汀有效抑制组胺诱导的IL-6、IL-8和GM-CSF分泌，平均半数抑制浓度（IC50）值分别为2.23、3.42和1.50纳摩尔。雷尼替丁和硫代哌酰胺在很宽的剂量范围内未能抑制细胞因子分泌。这些数据表明，肥大细胞衍生的组胺可能通过激活上皮细胞H1受体刺激过敏性结膜炎中炎性细胞因子的产生。组胺H1拮抗剂依美斯汀可有效抑制这种反应。
