伊曲康唑对小鼠巨噬细胞的形态学影响。

Morphological effects of itraconazole on murine macrophage.

作者信息

Ochi H, Ishijima S A, Abe S, Tameike A, Yamaguchi H, Osumi M

机构信息

Division of Human Development, Graduate School of Human Life Science, Japan Women's University, Tokyo.

出版信息

FEMS Immunol Med Microbiol. 1998 Mar;20(3):181-9. doi: 10.1111/j.1574-695X.1998.tb01126.x.

DOI:10.1111/j.1574-695X.1998.tb01126.x

PMID:9566489

Abstract

Morphological effects of itraconazole (ITCZ) on murine macrophages were examined by light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and compared with the effects of other antifungal agents. Thioglycolate-induced peritoneal macrophages were prepared from C3H/He J mice and cultured for 20 h in the presence of the antifungal azoles econazole, fluconazole, miconazole, ketoconazole, ITCZ, hydroxy-itraconazole (ITCZ-OH), and a polyene antibiotic amphotericin B (AMPH). Among these reagents, only ITCZ and its derivative ITCZ-OH were effective in causing morphological changes of murine macrophage as determined by LM and SEM. Macrophages treated with 2 microg/ml ITCZ or ITCZ-OH were stretched out bidirectionally, their surface was smooth and their 'ruffles' decreased. TEM observation showed that the bundles of the filamentous structure existed along the cell shape in the cytoplasm. These findings suggest that ITCZ and ITCZ-OH affect the morphology of macrophages.

摘要

通过光学显微镜（LM）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究了伊曲康唑（ITCZ）对小鼠巨噬细胞的形态学影响，并与其他抗真菌药物的作用进行了比较。从C3H/He J小鼠制备巯基乙酸盐诱导的腹腔巨噬细胞，并在抗真菌唑类药物益康唑、氟康唑、咪康唑、酮康唑、ITCZ、羟基伊曲康唑（ITCZ-OH）以及多烯抗生素两性霉素B（AMPH）存在的情况下培养20小时。在这些试剂中，通过LM和SEM测定，只有ITCZ及其衍生物ITCZ-OH能有效引起小鼠巨噬细胞的形态变化。用2μg/ml ITCZ或ITCZ-OH处理的巨噬细胞双向伸展，其表面光滑且“皱褶”减少。TEM观察显示，丝状结构束沿细胞质中的细胞形状存在。这些发现表明ITCZ和ITCZ-OH会影响巨噬细胞的形态。