Wang S, Millard W J, Meyer E M
Department of Pharmacology, University of Florida, Gainesville 32610, USA.
Neurochem Res. 1998 May;23(5):779-86. doi: 10.1023/a:1022467628383.
NGF expression in COS cells when driven by pTR.NGF (CMV promoter, AAV TRs) was more effective than either pc.NGF (CMV promoter, no AAV TRs) or dlk.NGF (AAV promoters and TRs). This NGF was able to differentiate PC12 cells. Differentiated PC12 cells transfected with pTR.NGF released NGF into medium. The fraction of pTR.NGF transfected PC12 cells that extended neurite-like processes 7 days post-transfection was similar to the transfection efficiency, suggesting that transfected cells were selectively differentiated by locally released NGF. pTR.NGF-transfected primary cultures of either neurons or glia did not express exogenous NGF. These results indicate that NGF can be released by dividing and non-dividing cells, but not neonatally derived brain cells.
当由pTR.NGF（巨细胞病毒启动子，腺相关病毒TRs）驱动时，NGF在COS细胞中的表达比pc.NGF（巨细胞病毒启动子，无腺相关病毒TRs）或dlk.NGF（腺相关病毒启动子和TRs）更有效。这种NGF能够使PC12细胞分化。用pTR.NGF转染的分化PC12细胞将NGF释放到培养基中。转染后7天延伸出类神经突样突起的pTR.NGF转染PC12细胞的比例与转染效率相似，这表明转染细胞被局部释放的NGF选择性地分化。pTR.NGF转染的神经元或神经胶质原代培养物不表达外源性NGF。这些结果表明，NGF可由分裂和非分裂细胞释放，但不能由新生脑源性细胞释放。
