一项使用[3H]胸腺嘧啶核苷对淋巴细胞中的DNA合成进行测定并收获到玻璃纤维滤盘上的评估。
An evaluation of an assay for DNA synthesis in lymphocytes with [3H]thymidine and harvesting on to glass fibre filter discs.
作者信息
Ahern T, Taylor G A, Sanderson C J
出版信息
J Immunol Methods. 1976;10(4):329-36. doi: 10.1016/0022-1759(76)90027-2.
Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 mjCi or [3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h.
使用半自动收获仪，对不同等级的玻璃纤维滤纸收集氚标记细胞的效果进行了比较。尽管发现GF/B滤纸对全血培养有用，但仍选择了GF/C级（Whatman有限公司）滤纸用于常规操作。文中讨论了影响胸苷浓度和活性选择的因素，结果表明，1 mCi的[³H]胸苷（5 Ci/mmol）/0.2 ml培养物与长达22小时的总DNA合成呈线性关系。
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