Wani G, Milo G E, D'Ambrosio S M
Division of Radiobiology, College of Medicine, Ohio State University, Columbus 43210, USA.
Cancer Lett. 1998 Mar 13;125(1-2):123-30. doi: 10.1016/s0304-3835(97)00507-7.
The expression of the 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase) gene in human breast tumors was evaluated at the level of the single cell to better understand how breast tumor cells regulate expression in response to oxidative stress. Compared to normal breast ductal cells, the level of 8-oxo-dGTPase expression in the breast tumor cells increased from non-detectable levels in normal breast to expression in 30-85% of the tumor cells in individual tumors. There was no significant association between 8-oxo-dGTPase expression and tumor grade and metastatic malignancy. The upregulation of 8-oxo-dGTPase was not directly linked to the expression of cyclins D1 and D3, estrogen receptor, p53, Ki-67 and c-erbB-2, which are genes involved in cell cycle regulation and tumor growth. The elevated expression of 8-oxo-dGTPase in human breast ductal carcinoma cells appears to be a general characteristic of breast tumors and may provide the tumor cell with a cellular defense mechanism to prevent the incorporation of 8-hydroxy-deoxyguanosine during DNA replication.
为了更好地理解乳腺肿瘤细胞如何响应氧化应激调节8-氧代-7,8-二氢脱氧鸟苷三磷酸酶（8-氧代-dGTPase）基因的表达，在单细胞水平对人乳腺肿瘤中该基因的表达进行了评估。与正常乳腺导管细胞相比，乳腺肿瘤细胞中8-氧代-dGTPase的表达水平从正常乳腺中无法检测到的水平增加到个别肿瘤中30%-85%的肿瘤细胞有表达。8-氧代-dGTPase的表达与肿瘤分级和转移恶性程度之间无显著关联。8-氧代-dGTPase的上调与细胞周期蛋白D1和D3、雌激素受体、p53、Ki-67和c-erbB-2的表达无直接联系，这些基因参与细胞周期调控和肿瘤生长。人乳腺导管癌细胞中8-氧代-dGTPase的表达升高似乎是乳腺肿瘤的一个普遍特征，可能为肿瘤细胞提供一种细胞防御机制，以防止在DNA复制过程中8-羟基脱氧鸟苷的掺入。
