Hagen M, Pabel U, Landsiedel R, Bartsch I, Falany C N, Glatt H
German Institute of Human Nutrition, Potsdam-Rehbrücke.
Chem Biol Interact. 1998 Feb 20;109(1-3):249-53. doi: 10.1016/s0009-2797(97)00136-1.
Various human sulfotransferases (hP-PST, hM-PST, hHST) and rat sulfotransferases (rPST-IV, rHSTa) have already been expressed in Ames' Salmonella strains (in particular in TA1538). Now a further strain, TA1538-hEST, which expresses the human estrogen sulfotransferase (hEST), has been constructed. This strain activated the primary benzylic alcohol 1-hydroxymethylpyrene (1-HMP) and the secondary benzylic alcohol 1-hydroxyethylpyrene (1-HEP) to mutagens. Human sulfotransferases hEST and hHST both activated 1-HEP, but they differed substantially in their enantioselectivity for this compound.
多种人类磺基转移酶（hP-PST、hM-PST、hHST）和大鼠磺基转移酶（rPST-IV、rHSTa）已在艾姆斯沙门氏菌菌株（特别是TA1538）中表达。现在构建了一种进一步的菌株TA1538-hEST，其表达人类雌激素磺基转移酶（hEST）。该菌株将伯苄醇1-羟甲基芘（1-HMP）和仲苄醇1-羟乙基芘（1-HEP）激活为诱变剂。人类磺基转移酶hEST和hHST都激活了1-HEP，但它们对该化合物的对映选择性有很大差异。
